PURPOSE To determine whether retinal neurons become more susceptible to injury by nitric oxide (NO) under hypoxic conditions. METHODS Cells from the RGC-5 line were exposed to different concentrations (0.1-100 microM) of S-nitroso-N-acetyl-penicillamine (SNAP), an NO donor, under normoxic and hypoxic (1.0% O(2)) conditions with 5.5 mM glucose or with no glucose. In some experiments, carboxy-P...

Intracellular vesicular trafficking and membrane fusion are important processes for nervous system development and for the function of neural circuits. Synaptosomal-associated protein 25 kDa (SNAP-25) is a component of neural soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) core complexes that mediate the exocytotic release of neurotransmitters at chemical synapses....

The effect of nitric oxide (NO) on Na+/H+ exchange (NHE) activity was investigated utilizing Caco-2 cells as an experimental model. Incubation of Caco-2 cells with 10(-3) M S-nitroso-N-acetylpenicillamine (SNAP), a conventional donor of NO, for 20 min resulted in a approximately 45% dose-dependent decrease in NHE activity, as determined by assay of ethylisopropylamiloride-sensitive 22Na uptake....

La investigación hace un recorrido desde la creación de Bellavista en década los noventa, como una iniciativa que combina conservación y el ecoturismo, hasta su declaratoria primera área protegida privada del Sistema Nacional Áreas Protegidas (SNAP) 2019, siguiendo procedimiento técnico-normativo establecido por Autoridad Ambiental. El objetivo fue documentar proceso llevó a propietarios conser...

A snap-stabilizing protocol guarantees that the system always behaves according to its specification provided some processor initiated the protocol. We present how to snap-stabilize some important protocols, like Leader Election, Reset, Snapshot, and Termination Detection. We use a Snap-stabilizing Propagation of Information with Feedback protocol for arbitrary networks as the key module in the...

We have examined the effect of alpha-SNAP on exocytosis in adrenal chromaffin cells by direct assay of exocytosis using patch-clamp capacitance analysis. Cells were recorded using the whole cell patch-clamp configuration and the cells dialysed with control pipette solution or with a pipette solution containing alpha-SNAP or the deletion mutant alpha-SNAP(41-295). The deletion mutant was found t...