In previous studies, the enzymatic conversion of phosphatidylglycerol to cardiolipin (diphosphatidylglycerol) in cell-free preparations from E. coli was shown to be stimulated by the addition of CDP-dipalmitin, suggesting the participation of the cytidine coenzyme as phosphatidyl donor. The present communication, however, presents three lines of evidence supporting the following mechanism for t...

We have studied the capacity of human neutrophils to release arachidonic acid from diacylglycerol, employing 1-stearoyl-2-[1-14C]arachidonoyl-sn-glycerol and 1-[1-14C]stearoyl-2-arachidonoyl-sn-glycerol as exogenous substrates. We have found that arachidonic acid is removed from diacylglycerol by the sequential action of two enzymes. First, the sn-1 position is split by 1-diacylglycerol lipase ...

Growth of Methanococcus jannaschii over a wide temperature range (47 to 75 degrees C) is correlated with an ability to alter dramatically the proportions of three ether lipid cores. These lipids shifted from predominantly diether (2,3-di-O-phytanyl-sn-glycerol) at the lower growth temperatures to macrocyclic diether and tetraether at near optimal growth temperatures. Lipid head groups varied as...

Glycerol kinase (8.C. 2.7.1.30, ATP: glycerol-3-phosphotransferase) catalyzes the phosphorylation of 28 nitrogen, sulfur, and alkyl-substituted analogues of glycerol. A survey of 66 analogues has defined qualitatively the structural characteristics nffissary for acceptability as a substrate by this enzyme. This survey was conducted using an assay based on 3lP NMR spectroscopy which detected pro...

During neonatal development glucocorticoids potentiate oligodendrocyte differentiation and myelinogenesis by regulating the expression of myelin basic protein, proteolipid protein, and glycerol phosphate dehydrogenase (sn-glycerol-3-phosphate: NAD+ 2-oxidoreductase, EC 1.1.1.8). The actual locus at which hydrocortisone exerts its developmental influence on glial physiology is, however, not well...

Glycerol Electrooxidation Reaction (GEOR) has been herein investigated on Rh/C and Rh/SnO 2 -C prepared by polyol method. The particle mean sizes were found to be 2.0 1.8 nm in -C, respectively. alloying degree reached 63% confirming a Sn-Rh alloy formation. activity towards GEOR was almost 5-fold higher than Rh/C, as demonstrated electrochemical measurements alkaline medium. This trend indicat...

Three distinct fractions containing polar glycolipids (PF1–3) were isolated from the chloroform soluble fraction of crude methanolic extract of red alga Chondria armata (Kütz.) Okamura on gel chromatography over Sephadex LH20. Their structure was elucidated by multidimensional nuclear magnetic resonance (NMR) techniques like 1H, 1H correlation spectroscopy (COSY), 1H, 1H total COSY (TOCSY), 1H,...