Idiopathic dilated cardiomyopathy (IDCM) is a frequent cause of heart transplantation. Potentially valuable blood markers are being sought, and low-density lipoprotein receptor-related protein 1 (LRP1) has been linked to the underlying molecular basis of the disease. This study compared circulating levels of soluble LRP1 (sLRP1) in IDCM patients and healthy controls and elucidated whether sLRP1...

BioSAXS is a popular technique used in molecular and structural biology to determine the solution structure, particle size shape, surface-to-volume ratio conformational changes of macromolecules macromolecular complexes. A high quality SAXS dataset for modeling must be from monodisperse, homogeneous samples this often only reached by combination inline chromatography immediate measurement. Most...

The NISTmAb is a monoclonal antibody Reference Material from the National Institute of Standards and Technology; it is a class-representative IgG1κ intended to serve as a pre-competitive platform for harmonization and technology development in the biopharmaceutical industry. The publication series of which this paper is a part describes NIST's overall control strategy to ensure NISTmAb quality ...

In an interlaboratory study we compare different methods to determine the weight-average molecular weight (Mw) and distribution of six cereal beta-glucan isolates nutritional importance. Size-exclusion chromatography (SEC) with multi-angle light scattering (MALS), capillary viscometry, sedimentation velocity analytical ultracentrifugation one asymmetric flow field-flow fractionation (AF4)-MALS ...

Protein dynamics play a significant role in many aspects of enzyme activity. Monitoring structural changes and aggregation biotechnological enzymes under native conditions is important to safeguard their properties function. In this work, the potential asymmetrical flow field-flow fractionation (AF4) study dynamic association equilibria β-D-galactosidase (β-D-Gal) was evaluated. Three commercia...

In aquatic environments, the term humic substances (HS) encompasses terrestrial and autochthonous refractory organic matter. HS are one of main fractions natural matter important chelators trace elements. environmental studies, determination is often restricted to dissolved fraction, content in suspended particles remains unknown. this work, we present composition dynamics along mixing gradient...

Herein, a more practical and efficient synthesis protocol for the preparation of uniform rod-like oligo(1,4-phenylene ethynylene)s (OPE)s is presented. Applying an iterative reaction cycle consisting decarboxylative coupling saponification alkynyl carboxylic ester, pentamer obtained in ten steps with 14% overall yield. The copper-free conditions prevent homocoupling until trimer stage, resultin...

The chemical nature of most organic P (Porg) in soil remains ‘unresolved’ but is accounted for by a broad signal the phosphomonoester region solution 31P nuclear magnetic resonance (NMR) spectra. molecular size range this NMR and its structure remain unclear. aim study was to elucidate Porg with increasing extracts combining exclusion chromatography (SEC) spectroscopy. Gel-filtration SEC carrie...

Extracellular vesicles (EV) are important for delivering biologically active substances to facilitate cell-to-cell communication. Milk-derived EV widely known because of their potential immune enhancement. However, procedures isolating milk-derived have not been fully established. To obtain pure and accurately reveal function, such must be The aim the present study was compare methods using com...

The characterisation of polymeric materials in their full complexity chain length, monomeric composition, branching and functionalization is a tremendous challenge best tackled by tailored multi-dimensional coupled analytical detection techniques. Herein, we focus on the improvement an affordable but information rich 2D-method for polymer analysis: online hyphenation benchtop 1H NMR spectroscop...