Genetic similarities and distances between wild-type olives in Turkey were studied using an RAPD-PCR assay. Seven wild olive tree samples were collected from villages in Manisa and Izmir provinces. Genomic DNA was extracted from young leaves and the RAPD-PCR assay was used to generate RAPD markers. Sixty-five random primers obtained from Operon Technologies were tested for the assay (OP-A 1-20,...

There is scanty information on tamarind’s genetic diversity in Easter Kenya. The objective of this study was to determine the 64 tamarind accessions from Eastern Kenya using 12 Inter Simple Sequence Repeat (ISSR) markers. DNA extracted young apical leaves modified CTAB method and amplified standard PCR. data collected were scored as presence (1) or absence (0) bands then compared 100bp ladder a...

Cannabis sativa L. (Cannabaceae) is one of the earliest known cultivated plants and is important in the global economy today as a licit and an illicit crop. Molecular markers distinguishing licit and illicit cultivars have forensic utility, but no direct comparison of hemp and marijuana amplified fragment length polymorphism (AFLP) has been made to date. Genetic variation was surveyed in three ...

Electrophoretic expression of 20 enzymes coded by a minimum of 30 gene loci is reported for sea lamprey. ADA, ADH. AGP, AAT, CPK, G6PDH, GAPDH, LDH. MDH, PEP, 6PCD, PGI, PGK, PGM, PK, PMI, and SDH yielded gel resolution satisfactory for use in future population studies. Six loci, AGP, !LZDH-1, PGI-I, PGI-2, PGi%f-l, and PlMI were polymorphic. Enzyme expresion did not vary between life stages (a...

We report sets of precise and reproducible measurements on the static pressure at the bottom of a granular column. We make a quantitative analysis of the pressure saturation when the column height is increased. We evidence a great sensitivity of the measurements with the global packing fraction and the eventual presence of shear bands at the boundaries. We also show the limit of the classical J...

background :  sequence-based identification of various microorganisms including archaea, bacteria, cyanobacteria, diatoms, fungi, and green algae necessitates an efficient and reproducible genome extraction procedure though which a pure template dna is yielded and it can be used in polymerase chain reactions (pcr). considering the fact that dna extraction from these microorganisms is time consu...

study of genetic relationships is a prerequisite for plant breeding activities as well as for conservation of genetic resources. in the present study, genetic diversity among and within 15 iranian native ajowan(carum copticum l.) populations were determined using inter simple sequence repeat (issr) markers. twelve selected primers produced 153 discernible bands, with 93 (60.78%) being polymorph...

Jacaranda decurrens (Bignoniaceae) is an endemic species of the Cerrado with validated antitumoral activity. The genetic diversity of six populations of J. decurrens located in the State of São Paulo was determined in this study by using molecular markers for randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP). Following optimization of the amplification ...