O extrato diclorometânico do tronco de Clusia melchiorii, após fracionamento e purificação por cromatografia em gel de sílica, produziu um novo bifenilo, 2,2-dimetil-5-hidroxi-7fenilcromeno, ao lado de outros compostos conhecidos, 2,2-dimetil-5,10-diidro-2Hbenzo[g]cromeno-5,10-diona (xiloidona), ácido betulínico, friedelina, friedelinol, eufol, sitostenona, estigmastenona e uma mistura de β-sit...

Medicinal plants in genus Lagerstroemia were investigated for phytochemical contents by GC-MS and HPLC with ethanol and hexane extracts and their toxicity MTT and comet assay on human peripheral blood mononuclear cells (PBMCs). γ-Sitosterol is the major component found in all species at 14.70-34.44%. All of the extracts, except for L. speciosa ethanol extract, showed high percentages of cell vi...

Bioassay-guided fractionation of an EtOAc extract of the stem wood of Euphorbia quinquecostata using a phorbol dibutyrate receptor-binding assay system as a monitor yielded four inhibitory isolates (1-4), inclusive of one new compound, 17-hydroxyingenol 20-hexadecanoate (1), and three previously known compounds, ingenol 20-hexadecanoate (2), ent-16 alpha,17-dihydroxyatisan-3-one (3), and ent-3 ...

N-Substituted succinamic acid beta-sitosteryl ester derivatives were prepared and evaluated. Compounds 1 and 2 were prepared in 76-92% yields by the reaction of beta-sitosterol and succinic anhydride, followed by the activation of the resulting acid compound 1 by thionyl chloride or methyl chloroformate, and finally by amination with appropriate amines. Compound 2a (DANA87) was also easily obta...

In the past few years, rapid strides have been made in our knowledge of the origin of the extra methyl and ethyl groups in the side chain of plant sterols. It now appears that desmosterol (3) (fig 1) or another A24 sterol is the recipient of a methyl group from S-adcenosyl methionine resulting in the formation of 24-methylene cholesterol (III) which is the receptor of another methyl group to fo...

Although abnormal liver function tests occur in 50-80% of cases with Turner syndrome, there are no previous reports of overt hepatic disease or hepatic granulomas associated with Turner's syndrome. We report three cases of Turner syndrome associated with hepatic granulomas with a wide range of liver dysfunction. Of the three patients, first patient underwent liver transplantation; second patien...

An analogous series of dimethylalkyl compounds, consisting of four amines, an amide, and a phosphonate ester, inhibited motility and reproduction of the nematode Caenorhabditis elegans. Dimethylamines with straight-chain lengths of 12, 14, or 16 carbon atoms were equally active nematicides, causing greater than 80% population growth inhibition at a concentration of 25 ppm. The C12 straight-chai...

Phytoestrogens are produced by plants and may cause endocrine disruption in vertebrates. The present study hypothesizes that phytoestrogen exposure of female Siamese fighting fish (Betta splendens) may disrupt endogenous steroid levels, change agonistic behavior expression, and potentially also disrupt oocyte development. However, only the pharmacologic dose of β-sitosterol had a significant ef...

Here we use nuclear magnetic resonance to measure the solubility limit of several biologically relevant sterols in electroformed giant unilamellar vesicle membranes containing phosphatidylcholine (PC) lipids in ratios of 1:1:X DOPC:DPPC:sterol. We find solubility limits of cholesterol, lanosterol, ergosterol, stigmasterol, and β-sitosterol to be 65-70%, ~35%, 30-35%, 20-25%, and ~40%, respectiv...

A new abietene diterpene, kaempfolienol (5S,6S,7S,9S,10S,11R,13S-abiet-8(14)-enepenta-6,7,9,11,13-ol, 1), was isolated from a rhizome extract of Kaempferia angustifolia Rosc. along with the known compounds crotepoxide, boesenboxide, zeylenol, 2'-hydroxy-4,4',6'-trimethoxychalcone, (24S)-24-methyl-5α-lanosta-9(11),25-dien-3β-ol, β-sitosterol and β-sitosterol-3-O-β-D-glucopyranoside. The structur...