BACKGROUND Polymerase chain reaction (PCR) is used to detect viruses in the cerebrospinal fluid (CSF) of patients with neurological disease. However, data to assist its use or interpretation are limited. OBJECTIVE We investigated factors possibly influencing viral detection in CSF by PCR, which will also help clinicians interpret positive and negative results. METHODS CSF from patients with...

Abstract Background Bacterial canker and subsequent gummosis are caused by multiple pathogens lead to significant yield productivity losses in sweet cherry cultivation Turkey. This study identified that Pseudomonas syringae pathovars were responsible for bacterial on orchards using classical molecular methods evaluated the biocontrol effects of bacteriophages against P. pv. syringae. Results Pa...

Although zinc ions have a strong antiviral impact against viral infections, they high level of toxicity in cell culture and animal models. In the current work, ZnO nanoparticles (ZnO NPs) fibrillar chitosan-ZnO nanostructures (CS-ZnO NSs) were synthesized order to evaluate their cytotoxicity inhibitory HSV (herpes simplex virus) replication vitro using MTT, plaque, Real-time PCR methods. Also, ...

In this study, the authors examined the technical performance of culture methodology using specific media: Mycoplasma isolation media of pleuropneumonia-like organisms (PPLO) broth and PPLO agar. Digitonin sensitivity, growth inhibition, the serum plate agglutination test, a commercially available enzyme-linked immunosorbent assay (ELISA) and a commercially available simplex polymerase chain re...

Herpes simplex virus (HSV) hepatitis by definition constitutes disseminated herpes simplex infection; it is rare, with only approximately 130 cases reported in the literature. Although HSV hepatitis typically occurs in immunocompromised hosts, pregnancy-especially the third trimester, has been identified as a risk factor for its development. This is likely because of the fact that humoral and c...

BACKGROUND Virus isolation in cell culture is the recognised diagnostic gold standard for genital herpes. Although increasing evidence indicates that polymerase chain reaction (PCR) provides a more rapid and sensitive diagnostic method, its implementation in routine diagnostic settings has been limited by concerns over contamination and cost. OBJECTIVE To evaluate the feasibility of replacing...

We consider polygon and simplex equations, of which the simplest nontrivial examples are pentagon (5-gon) Yang–Baxter (2-simplex), respectively. examine general structure (2n + 1)-gon 2n-simplex equations in direct sums vector spaces. Then, we provide a construction for their solutions, parameterized by elements Grassmannian Gr(n 1, 2n 1).

We evaluated a one-tube multiplex real-time PCR targeting DNA of Schistosoma haematobium complex and S. mansoni in serum samples obtained at different German diagnostic centers. Simplex protocols for the detection multi-copy DNA-repeats Dra1 Sm1-7 were combined to new format. The was subjected full validation including evaluation real-life setting with travelers migrants. results compared those...

background and aims: several drugs are being used in treatment of hsv infection in human but still introducing an effective safe drug is desirable. methods: we investigated the inhibitory effect of morphine on replication of hsv in vitro. results: the results indicated that a concentration of up to 200 ug/ml morphine had a limited effect on vero cell viability. at this concentration the growth ...