Most multicellular organisms have a capacity to regenerate tissue after wounding. Few, however, have the ability to regenerate an entire new body from adult tissue. Induction of new shoot meristems from cultured root explants is a widely used, but poorly understood, process in which apical plant tissues are regenerated from adult somatic tissue through the de novo formation of shoot meristems. ...

In an effort to elaborate the elements of actinidia micropropagation methodology, 2 varieties male form
 (Kamandor, Pryvabny) and female form (Sentyabrskaya, Prevoshodnaya) were selected. The shoot tips forms
 buds lignified shoots forms used as initial explants. regenerative ability explants at stage in vitro culture induction was defined by plant genotype type explant selected: expl...

Cotton (Gossypium spp.) belonging to the genus Gossypium in the family Malvaceae. Cotton is an important fibre crop of global importance. It is grown in tropical and subtropical regions of more than 80 countries with an annual production of 20 million tonnes. It is an important source of oil and high quality protein meal and plays a significant role in the national economy. Among the cotton pro...

Shoots can be regenerated from Arabidopsis (Arabidopsis thaliana) root explants in tissue culture through a two-step process requiring preincubation on an auxin-rich callus induction medium. Regenerating tissues can be directed along different developmental pathways leading to the formation of shoots, new roots, or callus by transferring to the appropriate organ induction medium. Using gene-pro...

Pueraria candollei Wall. ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66-100% of leaf or stem explants formed calli. Calli from stem explants cultured on MS medium supplemented with 0.5 mg/l thidiazuron (TDZ) gave the maximum of shoot indu...

In vitro direct regeneration of Nelumbo nucifera Gaertn. was successfully achieved from immature explants (yellow plumule) cultured on a solid MS media supplemented with combinations of 0.5 mg/L BAP and 1.5 mg/L NAA which resulted in 16.00 ± 0.30 number of shoots per explant and exhibited a new characteristic of layered multiple shoots, while normal roots formed on the solid MS basal media. The...

An efficient variety-independent method for producing transgenic eggplants (Solanum melongena L.) via Agrobacterium tumefaciens-mediated genetic transformation was developed. Two weeks old cotyledon explants were transformed by co-cultivation with Agrobacterium tumefaciens strain LBA4404 harboring a binary vector pVDH65 carrying the reporter gene β-glucuronidase intron (GUS-INT) and the marker ...

Withania somnifera (L.) Dunal is high demanding valuable medicinal plants which have pharmaceuticals properties, known for its with anolides. Nodal segment explants of W. somnifera when cultured on MS (Murashige and skoog’s, 1969) medium horizontally containing different concentration(1-3mg/l) of 6-benzylaminopurine (BAP), kinetin (Kn) and thidiazuron (TDZ) produced multiple shoots on both axil...

A reliable in vitro propagation protocol has been established from nodal segments of a highly valuable medicinal plant Solidago virgaurea L. The explants were cultured on Murashige and Skoog’s medium augmented with different concentrations and combinations of plant growth regulators for shoot bud initiation and multiplications. Highest frequency of shoot proliferation (91.8 ± 0.64) and maximum ...

Highly efficient in vitro regeneration system has been developed for Swertia lawii Burkill, an important herb used as substitute for Swertia chirayita. Shoot tips explants were cultured on MS medium with various phytohormones for multiple shoot production. The best shoot production frequency (100%) and maximum shoots (10.4 ± 0.8) were obtained on MS media containing TDZ (3.0 mg l-1) in combinat...