H(2) generated from renewable resources holds promise as an environmentally innocuous fuel that releases only energy and water when consumed. In biotechnology, photoautotrophic oxygenic diazotrophs could produce H(2) from water and sunlight using the cells' endogenous nitrogenases. However, nitrogenases have low turnover numbers and require large amounts of ATP. [FeFe]-hydrogenases found in oth...

This work reported on the in vitro antibacterial activity of six selected marine algae (seaweeds) which have been selected and their extracts have been tested as an alternative to commonly used antibiotics. Extracts of six seaweed samples namely Codium decorticatum, Caulerpa scalpelliformis, Gracilaria crassa, Acanthophora spicifera, Sargassum wightii and Turbinaria conoides were collected from...

A chromosome-encoded beta-lactamase gene from a Shewanella oneidensis reference strain was cloned and expressed in Escherichia coli. It encoded a carbapenem-hydrolyzing Ambler class D beta-lactamase, OXA-54, that shared 92% amino acid identity with the plasmid-encoded carbapenem-hydrolyzing oxacillinase OXA-48 from Klebsiella pneumoniae. This work suggests that Shewanella spp. may produce the p...

The marine bacterium Vibrio tapetis is the causative agent of the brown ring disease, which affects the clam Ruditapes philippinarum and causes heavy economic losses in North of Europe and in Eastern Asia. Further characterization of V. tapetis isolates showed that all the investigated strains harbored at least one large plasmid. We determined the sequence of the 82,266 bp plasmid pVT1 from the...

The recent discovery of electrically conductive bacterial appendages has significant physiological, ecological, and biotechnological implications, but the mechanism of electron transport in these nanostructures remains unclear. We here report quantitative measurements of transport across bacterial nanowires produced by the dissimilatory metal-reducing bacterium, Shewanella oneidensis MR-1, whos...

*Correspondence: Fengping Wang, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China e-mail: [email protected] †Present address: Ying Chen, Department of Biochemistry and Molecular Biology, Guilin Medical University, Guilin, China Shewanellae are well known for their ability to utilize a number of electron acceptors and are the...

Bioprospecting in unusual marine environments provides an innovative approach to search novel biomolecules with antibiofilm activity. Antarctic sponge-associated bacteria belonging Colwellia, Pseudoalteromonas, Shewanella and Winogradskyella genera were evaluated for their ability contrast the biofilm formation by Pseudomonas aeruginosa ATCC 27853 Staphylococcus aureus 29213, as model organisms...

Shewanella exhibit a remarkable versatility of respiration, with a diverse array of electron acceptors (EAs). In environments where these bacteria thrive, multiple EAs are usually present. However, we know little about strategies by which these EAs and their interaction affect ecophysiology of Shewanella. In this study, we demonstrate in the model strain, Shewanella oneidensis MR-1, that nitrit...