background and objectives: bordetella species colonize the respiratory tract of mammals and thereby cause the whooping cough. most of the species produce adenylate cyclase - a toxin ( hemolysin ) responsible for increasing intracellular cyclic amp (camp) levels in mammalian neutrophils and macrophages and as a consequence their phagocytic function get impaired . this study was carried out to is...

Peripheral blood mononuclear cells (PMC), isolated by density gradient techniques with Ficoll-Hypaque, contain Tand B-lymphocytes and monocytes. An/I hydrocarbon hydroxylase (AHH) activity was measured in PMC subtrac tions consisting of T-lymphocyte-enriched, T-lymphocytedepleted, and monocyte-depleted populations. The T-cellenriched populations consistently showed enhancement of AHH activity w...

Dose-survival curves were constructed for the effect of eight cytotoxic agents on both nonproliferating and prolifer ating bone marrow-derived lymphocytes (B cells). The system used was the primary response of AKR mice to sheep red blood cells. Cytotoxic agents were given either 24 hr before or 24 hr after the antigen stimulation. These times were chosen to represent a time when the majority of...

Phytohemagglutinin (PHA) stimulated the rate of DNA synthesis in rabbit spleen cell suspensions. Unlike antigens, previous immunization to PHA was not necessary and the specific response could not be transferred by macrophages, although lymphocytes primed by incubation in PHA were able to stimulate other spleen cells not directly exposed to PHA. When rabbits were stimulated by in vivo immunizat...

The complement system is a group of proteins that when activated lead to target cell lysis and facilitates phagocytosis through opsonisation. Individual complement components can be quantified however this does not provide any information as to the activity of the pathway. The CH50 is a screening assay for the activation of the classical complement pathway (Fig 1) and it is sensitive to the red...

AIM This investigation is placed in the context of continuity of a preliminary study already published; it was conducted in cattle, sheep, goats, horses, and dogs; the main aim is to reveal and develop criteria for the animal species determination based on the morphometric parameters of red blood cells. MATERIALS AND METHODS Blood samples were taken from the jugular vein; and the smears were ...