The elements of the mechanized fluorometric lipase (EC 3.1.1.3) assay of Fleisher and Schwartz [Clin. Chem. 17, 417 (1971)] were made optimum; a manual adaptation was also developed. Hydrolysis of the monodecanoyl fluorescein substrate by hog pancreatic lipase is a zero-order reaction. The hydrolytic activity of normal human serum, serum from hospitalized patients without pancreatitis, and seru...

We have previously shown that autonomous replication of human chromosome fragments is stimulated by the presence of an 18 bp sequence, REE1, which exhibits transcriptional silencer activity. The REE1 sequence is partly homologous with the serum response element (SRE) required for expression of the human c- fos gene. Here we have examined interaction of REE1 with human nuclear proteins using a g...

The influence of different copper and zinc contents in rations on blood serum concentrations in dependence on feeding (Groups A and B) and lactation (Lactation 1 and 2) was tested in a feeding trial with 60 German Holstein cows. All animals received a diet based on maize and grass silage ad libitum. 30 cows received a concentrate supplemented with copper and zinc as recommended (Group A), where...

The present study compared the levels of serum Mg, Cu and Zn between the adult with asthma and healthy volunteer in order to evaluate the associations of these nutrients with asthmatic patients. Thirty-three adults patients of both (19 males, 14 females) with the median age 39 years with bronchial asthma, who were admitted by the Department of Pulmonary Diseases of Shat and Alkyfaya Hospitals, ...

Background: Breast cancer is the most common serious disease around the world. The trace elements have a vital role in the metabolism and chemotherapy may change the level of metal ions. Due to the ambiguity of the existence in this regard, the study examined the trace element serum levels in women with breast cancer before and after chemotherapy . Methods: Sixty patients were studied undergoi...

Background: Oral lichen planus (OLP) is a common inflammatory disease of unknown origins. Recent studies have considered nutritional factors concerning the pathogenesis of many autoimmune disorders. The objective of the present study was to investigate the plasma levels of magnesium (Mg), calcium (Ca), zinc (Zn), copper (Cu), and iron (Fe) in this disorder. Methods: Included in this observation...

Background: the supplementation of natural antioxidants such plant flavonoids may counteract the deleterious actions of gamma-irradiation and iron overload. Material and Methods: the protective effect of naringin (NIN) against kidney injury induced by γ-irradiation (IRR) (6.5 Gy) and/or iron dextran (ID) (50 mg/kg. b. w) has been studied in rats given 80 mg/kg b.w of NIN for 14 days before, and...

The study was aimed to evaluate the influence of the vascular disease, atherosclerotic obliterans (AO), on the location and concentration of elements in the arterial wall and serum. Use of a modern method for studying element's concentration and distribution in samples of clinical material, i.e. laser ablation inductively coupled plasma mass spectrometry, is presented. Elements are not equally ...