The identification and the establishing of relationships among the various antigens associated with the mycobacteria have been the subject of innumerable investigations. The classical serologic technqiues of complement-fixation, agglutination, and precipitation have been employed in this endeavor by many investigators. Information has also been provided by sensitizing both normal and tanned ery...

HLA-A24 is the second most frequently expressed HLA-A type in Koreans (GF 22.8%). Four different serologic reaction patterns were observed in Korean A24 positive samples using a commercial serologic typing kit. To clarify the nature of serologic heterogeneity, thirteen A24 positive DNA samples representing the four different serologic reaction patterns were subjected to DNA sequencing analysis ...

The Western blot method, using antigens from epimastigote forms of the Trypanosoma cruzi Y strain, was evaluated for the confirmatory diagnosis of Chagas' disease. Serum samples were obtained from 136 chagasic patients (Group I), 23 patients with inconclusive serologic results for Chagas' disease (Group II), 53 patients with other diseases (Group III), and 50 healthy individuals (Group IV). The...

Anaplasmosis and ehrlichiosis are emerging tick-borne diseases with clinically similar presentations caused by closely related pathogens. Currently, laboratories rely predominantly on blood smear analysis (for the detection of intracellular morulae) and on serologic tests, both of which have recognized limitations, for diagnostic purposes. We compared the performance of a published real-time PC...

The natural history of Ehrlichia chaffeensis, the causative agent of human monocytotropic ehrlichiosis, includes the lone star tick (LST, Amblyomma americanum) as a vector and white-tailed deer (WTD; Odocoileus virginianus) as both a natural reservoir of E. chaffeensis and a major host of LST. The goal of the current study was to implement and evaluate a prototype surveillance system to delinea...

Immunoglobulin A (IgA) deficiency occurs more frequently in patients with celiac disease (CD) than in the general population and can lead to false-negative results in the best serologic test for CD, endomysial IgA (EMA). To evaluate the impact of IgA deficiency on serologic detection of CD in a reference laboratory setting, IgA levels were measured in 510 consecutive serum specimens submitted f...

and commercial kits (3). Future availability of promising ultra-sensitive tests (e.g., PCR and antigenic tests) may overcome the limitations associated with conventional microscopy and serologic testing for low-parasite load schistosomiasis. As stated in our manuscript, we cannot exclude the possibility that our case definition generated false-positives; the potential limitations of our finding...

aim : the aim of this study was to determine the prevalence of celiac disease in iranian patients suffering from chronic depression or schizophrenia. background : psychiatric disorders are common in untreated celiac disease. patients and methods : two hundred iranian inpatient men with in chronic phase of depressive disorders or schizophrenia, and 200 age-matched healthy male subjects were scre...

Introduction Hansen’s disease, or leprosy, is a chronic bacterial infection that affects the nerves, skin, eyes, and nose lining. In 2019, there were 202,256 new cases reported globally, nearly 28,000 are diagnosed each year in Brazil. The best way to prevent spread of disease early diagnosis treatment infected individuals. Most diagnoses done clinically, but only microscopic analysis slit-skin...

Brucellosis is a disease of veterinary and public health importance worldwide. In sub-Saharan Africa, where the bacterium Brucella abortus has been identified in several free-ranging wildlife species, successful disease control may be dependent on accurate detection in wildlife reservoirs, including African buffalo (Syncerus caffer). We estimated the sensitivity and specificity of a commercial ...