All cells contain proteases, which effect catalytic hydrolysis of the peptide bond between amino acids in the protein backbone. Typically, proteinases are prevented from nonspecific proteolysis by regulation and physical separation into different subcellular compartments; however, this segregation is not retained during cell lysis to release a protein of interest. Prevention of proteolysis duri...

Cell separation techniques are used widely in immunology studies. The one-step antigen-specific separation technique on a microfluidic platform based on shear horizontal surface acoustic wave (SH-SAW) sensors. With the specific antibodies conjugated onto the surface of the SH-SAW sensors could be used to specifically recognize the specific cells in the clinical body fluids. The cell isolation a...

Glandular trichomes (GTs) are one of the epidermal tissues medicinal plants which function in synthesis, storage, and secretion secondary metabolites. The active ingredients Chinese materials mostly metabolites plants. Accordingly, it is great research value to explore quality using GTs as starting point. However, most current studies on still at simple morphological identification stage, there...

Since the 1990s, interest in natural product research has increased considerably. Following several outstanding developments in the areas of separation methods, spectroscopic techniques, and sensitive bioassays, natural product research has gained new attention for providing novel chemical entities. This updated review deals with sample preparation and purification, recent extraction techniques...

Within a few years affinity chromatography has become a widely used technique for the separation and purification of enzymes. The development and advantages of general ligands in affinity chromatography have previously been reported (Mosbach et al., 1972; Ohlsson et al., 1972). Thus Sepharose-bound N6-(6-aminohexyl)-AMP has been used for the separation and purification of NAD+-dependent enzymes...