The aim of this study was to explore the molecular profile of proliferating rheumatoid arthritis synovial fibroblasts (RA-SF). Total RNA was extracted from two cultures of RA-SF (low-density [LD] proliferating cells and high-density [HD] nonproliferating cells) and suppression subtractive hybridization was performed to compare differential gene expression of these two cultures. Subtracted cDNA ...

PURPOSE To identify differentially expressed genes in corneal neovascularization using cDNA macroarray. METHODS Mechanical denudation of corneal and limbal epithelium was performed to induce corneal vascularization in mice. Corneas were harvested 4 days after operation. Total RNA was isolated from both normal and vascularized corneas and used for the synthesis of cDNA probes. 32P labeled expo...

PURPOSE To identify lens epithelial genes with altered expression levels in age-related human cataract. METHODS Epithelia from age-related cataracts and from normal lenses were microdissected and RNA was extracted. RNAs were compared for gene expression differences by RT-PCR differential display. Transcripts exhibiting altered levels of gene expression were cloned and identified by sequencing...

We analyzed tachykinin NK(3) receptor (NK(3)R) gene expression by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) in uteri from young (3-month-old) and old (30-month-old) rats. In addition, we characterized the expression of the preprotachykinin-B (TAC-3) gene, which encodes neurokinin B (NKB), the preferred endogenous agonist of NK(3)R. Compared with young rats, NK(3)...

infectious bronchitis virus (ibv) is an acute and contagious viral disease of poultry that affects different systems, including the respiratory tract in particular. ibv causes major economic losses in the poultry industry globally. due to antigenic variation of the causative agent, control of the disease is difficult. to control the disease, many vaccines that belong to different serotypes are ...