Developing effective cooled semen protocols is essential to increase pregnancy rates and reproductive efficiency in donkeys. This study aimed evaluate the effect on sperm kinetic parameters membrane integrity donkey diluted with defined milk proteins extender 1% or 2% of egg yolk removal seminal plasma. Twenty-four ejaculates from six jackasses were collected. Each ejaculate was divided into fo...

ABSTRACT This study aimed to evaluate the addition of different concentrations IGF-I and insulin egg yolk-based extender improve bovine semen cryopreservation. Two experiments were developed effects additives in two commercial extenders, Botubov® (Experiment 1) Triladyl® 2), both with same design. Three ejaculates from four bulls (n = 12) used. Each ejaculate was divided into seven equal fracti...

The mobility of pooled turkey sperm following various storage regimens was assessed by objectively measuring the ability of sperm to penetrate a 2% Accudenz [5-(N-2,3-dihydroxypropylacetamido)-2,4,6-tri-iodo-N,N'-bis(2,3-dihydroxypropyl)isophthalamide] solution at 41 C. When semen was diluted with Beltsville poultry semen extender and stored at 5 C with agitation at 150 rpm, sperm mobility decl...

This study was carried out to investigate effects of copper sulphate (CuSO4) additive to semen extenders on sperm parameters: progressive motility, viability, membrane integrity and DNA damage, after semen dilution and cryopreservation. Semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions during the autumn 2011. A total number of 25 samples were u...

Campylobacter is one of the most commonly reported bacterial causes of human foodborne infections in the United States. Recent evidence has demonstrated that Campylobacter is present in poultry semen and may contribute to the vertical transmission between the breeder hen and offspring. As Campylobacter is considered sensitive to oxygen and cold temperature, the objective of this study was to de...

Oxidative stress during freezing-thawing reduces sperm motility, viability, membrane functions and antioxidant capacity. Thymus vulgaris has antioxidant properties due to phenolic compounds. The purpose of current study was to evaluate the effect of Thymus vulgaris extract as a natural antioxidant on post-thawed Moghani ram sperm quality. Semen samples were collected twice a week from four rams...

Dilution and extension of semen longevity has been widely used in the swine industry to improve reproductive efficiency and genetic progress through artificial insemination. Through the dilution and extension of the life of the sperm cell, other organisms such as bacteria are also maintained and can produce a negative impact on sperm cell motility and potential reproductive capacity. Bacterial ...

The comet assay, under neutral conditions, allows the assessment of DNA integrity influenced by sperm ageing, which is manifested in DNA double-strand breaks. Here, we attempted to use a modified neutral comet assay test (single-cell gel electrophoresis), to our knowledge for the first time, to assess DNA integrity of boar spermatozoa during liquid storage for 96 h at 5 ̊C and 16 ̊C. In this come...

The survivability of caprine spermatozoa was compared in semen extenders prepared from the conventional egg-yolk citrate and goat-milk citrate. Motility was comparable in both sets of extenders during the first 24 hours post-extension when extended ejaculates were stored at either room temperature (28 O C) or refrigerator temperature (5 O C). Motility in goat milk citrate extenders at 5 O C was...