This study was carried out to investigate effects of copper sulphate (CuSO4) additive to semen extenders on sperm parameters: progressive motility, viability, membrane integrity and DNA damage, after semen dilution and cryopreservation. Semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions during the autumn 2011. A total number of 25 samples were used in each...

In mares, a physiological endometritis ensues following the deposition of semen into the uterus. This response is thought to be due to the antigenic nature of spermatozoa, specifically the activation of complement, and may be modulated by the presence of seminal plasma component of the semen. The removal of seminal plasma during cryopreservation of spermatozoa may account for the longer duratio...

Caffeine was incorporated in egg yolk tris glycerol extender (EYTG) at the time of dilution of Murrah semen at a concentration of 1.0, 3.0 or 5.0 mM. The samples were subsequently frozen. The post thaw progressive motility and live sperm per cent improved signifi cantly (P<0.05) with addition of 1.0 (70.0±0.99 and 88.80±0.84) and 3.0 mΜ (65.83±0.88 and 86.16±0.96%) caffeine to the extender befo...

Cryopreservation is currently the only effective tool for long-term storage of semen in most species. However, it well-recognized that, even species that freeze well, some individuals resist cryopreservation. Work from this laboratory has demonstrated a relationship between maternal lipid content and chemical constitution embryos they produce. The objective present study was to determine if sim...

White rhinoceroses suffer from a low rate of reproduction in captivity. This study examines the role of male reproductive function as a contributing factor. We used ultrasonography to image accessory sex glands, testis and epididymis. Electroejaculation provided 36 ejaculates from 21 rhinoceroses. Based on the percentage of progressively motile spermatozoa, semen was categorized in three groups...

In this project transcriptome analysis on highthroughput RNA-sequencing (RNA-Seq) data of boar spermatozoa will be utilized to identify novel genetic markers associated with semen freezability. Three boars each with poor and good semen freezability will be subjected to transcriptome studies on sperm RNA-seq data, and in combination with bioinformatics screening, will select candidate genes asso...

The purpose of this study was to evaluate 2 methods of semen collection that could be used as terminal procedures in stallions with irreparable conditions, such as fractures or colic. Electroejaculation was attempted under general anesthesia. Forty-eight hours later, the ponies were castrated and 2 different epididymal sperm collection techniques were attempted by using a flushing or floating m...

Cryopreserved mammalian semen is generally acknowledged to have an impaired fertility by comparison with fresh semen. The reduction arises from both a lower viability post-thaw and sublethal dysfunction in a proportion of the surviving subpopulation. The reasons for the loss of fertility are various. In this paper, factors affecting the proportion of survivors (e.g., cold shock susceptibility, ...

A study was conducted to investigate the various glycerol concentrations on freezability of mithun semen. The present study was conducted in semen production laboratory, ICAR-NRC on Mithun, Medziphema, Nagaland, India. A total of 20 ejaculates were collected from apparently healthy mithun bulls (n=6) by trans-rectal massage method. These ejaculates were extended with tris–egg yolk-citrate-glyce...