introduction: shigella is a facultative intracellular pathogen that uses the host actin cytoskeleton protein for intra- and intercellular spread. the aim of this study was to determine the distribution of icsa gene and icsa expressed protein bands among shigella flexneri strains isolated from 3 clinical centers in tehran. material and methods: two hundred and seventy five isolated shigella flex...

Introduction: Bacteria in the genus Pasteurella live as commensal parasites on mucous membranes of vertebrates, particularly mammals and birds. species like multocida haemolytica cause significant economic losses a result diseases caused by these two microorganisms. Objective: This study compares protein isolation analysis methods for effective one two-dimensional gel electrophoresis multocidap...

Figure S1 (related to Figure 1). Biochemical and functional characterization of the purified IP 3 R1. (A) SDS-PAGE of the purified IP 3 R1 protein: Coomassie blue stained SDS-PAGE gel (left panel) and immunoblot with an IP 3 R1-specific antibody (right panel): lane 1 – molecular weight standards (kDa); lane 2 – ER microsomal membranes; lane 3 – the purified IP 3 R1. The band corresponding to th...

An alternative electrophoretic method that improves the separation, identification and relative quantification of bovine milk casein fractions and casein hydrolysates was developed. The method is based in the Tricine-SDS-PAGE technique while the relative quantification of milk proteins has been achieved by digital densitometry. The advantages of this procedure for milk proteins and milk protein...

background and objectives: sodium dodecyl sulfate (sds) is one of the most widely used anionic detergents. the present study deals with isolation and identification of sds-degrading bacteria from a detergent contaminated pond situated in varanasi city, india. materials and methods : employing enrichment technique in minimal medium (pbm), sds-degrading bacteria were isolated from pond water samp...