The cryo scanning electron microscopy (cryo-SEM) as well as high-pressure-freezing (HPF) belongs to cutting edge techniques in electron microscopy of hydrated samples [1]. However, their combination is not always easily applicable. Here, we present a way of combining high-pressure-freezing using EM PACT2 (Leica Microsystems) that fixes hydrated samples on a 1.4 mm sapphire discs and high resolu...

nanoparticles of mn0.5zn0.5-xcdxfe2o4 with x varying from x = 0.0 to 0.3 were prepared by wet chemical co-precipitation method. the structural and magnetic properties were studied by using x-ray diffraction (xrd), scanning electron microscope (sem), transmission electron microscopy (tem) and electron paramagnetic resonance (epr) technique. the lattice constant increases with increase in cd cont...

Recently, three-dimensional reconstruction of ultrastructure of the brain has been realized with minimal effort by using scanning electron microscopy (SEM) combined with focused ion beam (FIB) milling (FIB-SEM). Application of immunohistochemical staining in electron microscopy (EM) provides a great advantage in that molecules of interest are specifically localized in ultrastructures. Thus, we ...

Silica nanoparticles are imaged in solution with scanning transmission electron microscopy (STEM) using a liquid cell with silicon nitride (SiN) membrane windows. The STEM images reveal that silica structures are deposited in well-defined patches on the upper SiN membranes upon electron beam irradiation. The thickness of the deposits is linear with the applied electron dose. Scanning electron m...

Caffeine loaded magnetic nanoparticle was successfully synthesized and were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDX), thermo gravimetric analysis (TGA), differential thermal analysis (DTA) and vibrating sample magnetometer (VSM). The resulting nanocom...

The preparation of nanostructure type manganese oxide and cobalt oxide materials with the smallest particle size is reported here. The nanorod manganese oxide and cobalt oxide nanotube were prepared via a sol-gel reaction in reverse micelles from KMnO4 and CoCl2 with respectively source at room-temperature. The structure and surface morphology of the obtained manganese oxide were studied by mea...

Physical characterization of semiconductor device has always been a challenging task for device engineers and researchers. Up to now, standard methods for characterizing semiconductors did not provide an effective means for determining two-dimensional quantities of sub-device scale. These methods include Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Secondary Ion M...

We designed and built a combination of a scanning tunneling microscope ~STM! and a scanning electron microscope ~SEM! which is working under ultrahigh vacuum conditions ~base pressure typically 7 • 10 mbar!. The SEM is ideally used for surveying the sample and to control the STM tip positioning, while the STM extends the resolution range into the atomic scale. The design concept allows moving t...

Semithin sections are commonly used to examine large areas of tissue with an optical microscope, in order to locate and trim the regions that will later be studied with the electron microscope. Ideally, the observation of semithin sections would be from mesoscopic to nanoscopic scales directly, instead of using light microscopy and then electron microscopy (EM). Here we propose a method that ma...