Three new pentasaccharide resin glycosides, batatosides III-V (1-3), were isolated from the roots of Sweet potato (Ipomoea batatas). Saponification of the crude resin glycoside mixture yielded substituents and simonic acid B. The structures of the isolated compounds (1-3) were established through spectroscopic analyses, including high field NMR spectroscopy and HR-ESI-MS, and chemical correlati...

Rigorous alkaline hydrolysis of the two primary (cholic and chenodeoxycholic) and of the two preponderant secondary (deoxycholic and lithocholic) bile acids found in bile led to excellent recoveries. Such was not the case with 11 different keto bile acid standards. Recoveries for a number of standards were unacceptably low and a variety of artefactural products were tentatively identified by ga...

In 1848, Claude Bernard described the lipolytic function of the pancreas. His experimental procedure was original and as he always affirmed, easily replicable. In this study we repeated the original Bernardian experiments for emulsification and saponification of fats, following the original descriptions from his laboratory manuscripts. The results were astonishingly similar to those obtained by...

Glycerol is a valuable byproduct in biodiesel production by transesterification, soap manufacturing by saponification as well as hydrolysis reaction. The purity of glycerol obtained is low due to the presence of impurities such as remaining catalyst, water, soaps, salts and esters formed during the reaction. Purification of glycerol as well as the conversion of glycerol into valuable products h...

1. The total lipid was extracted from the non-capsulated strain ofPneumococcus I-192R, A.T.C.C. 12213, with chloroform-methanol mixtures. Two glycolipids were isolated by chromatography on silicic acid and DEAE-cellulose (acetate form). 2. The major glycolipid was obtained pure in a yield of 640mg./34g. dry wt. of cells and represents about 34% of the total lipid. It contained galactose, glucos...

Measurement of cholesterol is sufficiently important in the diagnosis of cardiovascular disease so that a reference method should be available in the laboratory for evaluation of the rapid screening procedures in common use. With this in mind, a critical evaluation of the Abell procedure was carried out. The ex­ traction procedure, the effect of interfering substances, the effect of time and te...