I . A technique for the separation and colorimetric estimation of z,4-diaminopimelic acid (DAP) using automated ion-exchange chromatography coupled with an acid ninhydrin detection system is described. 2. Only traces of DAP were found in rumen protozoa and no DAP was detected in rumen fluid prepared by ultracentrifugation or dialysis. 3. The concentration of DAP in rumen bacteria from sheep on ...

A mechanistic model describing rumen digestion is presented. The model consists of 22 compartments for classical nutrients, microbes associated with either solids or fluid, and 8 amino-acid compartments. An empirical approach for volatile fatty acids production is used. Sensitivity and behavioural analysis on several parameters demonstrated the need to improve knowledge concerning certain facto...

Genomic analysis of Clostridium sp. NCR, an anaerobic Gram positive bacterium which was isolated from rumen fluid of Mehsani breed of buffalo revealed presence of various environmental gene tags (EGTs) involved in pathways for utilizing a wide range of substrates. Here we report the sequence of this rumen isolate, its whole genome sequence has been deposited in DDBJ/EMBL/GenBank under the acces...

Three polycentric rumen fungi, LL, LC2 and Ruminomyces elegans (C2), isolated from the rumen of cattle were grown in six culture media. LL and LC2 were morphologically similar. Their characteristics resembled those of Orpinomyces and Neocallimastix joyonii, and they grew well and produced sporangia after 3-4 d growth in all the media. R. elegans differed morphologically from LL and LC2, but alt...

Story in Brief The phosphorus concentration in ruminal fluid was determined for steers limit-fed rations with phosphorus concentrations ranging from .06 to .17 percent. Ruminal phosphorus concentration was increased with phosphorus intake at dietary concentrations below .12 percent for steers. This relationship was employed to estimate the ruminal availability of phosphorus from four phosphorus...

Mature Angus steers were intraruminally infused with base to increase ruminal pH. Base infusions, increasing rumen pH from 5.8 to 6.2, did not change duodenal pH but tended to reduce fecal pH. Increased ruminal pH tended to increase digestion of fiber (9 percent) but reduced ruminal digestion of starch by 18 percent (P> .05). Ruminal pH and ruminal starch digestion were negatively correlated (P...

Current systems for the evaluation of protein-feeds for ruminants take account of two principal nitrogen (N) requirements. One is to satisfy the needs of the rumen micro-organisms in order to maximize. microbial protein yield this N can largely be provided in any form that will give rise to enough ammonia in rumen fluid. The other N requirement is for a dietary source of true protein that wi,ll...

1. Protease and deaminase activities and the metabolism of peptides were measured in rumen fluid from ciliate-free sheep and from sheep with a limited population of small entodinia. The same measurements were repeated following inoculation of the latter group with a more typical mixed ciliate population. 2. Protease and dialanine uptake activities of mixed rumen micro-organisms were not signifi...

DEHORITY, B. A. (Ohio Agricultural Research and Development Center, Wooster). Characterization of several bovine rumen bacteria isolated with a xylan medium. J. Bacteriol. 91:1724-1729. 1966.-Studies were conducted to characterize eight strains of bacteria isolated from bovine rumen contents, by use of a medium containing xylan as the only added carbohydrate source. Based on morphology, biochem...