avian influenza (ai) is a viral respiratory disease of domestic and wild birds. in the diagnostic laboratory, it is essential to have methods for rapid detection of avian respiratory viruses. cloacal swabs collected from chickens experimentally infected with h9 subtype ai virus, used in a reverse transcription-polymerase chain reaction (rt-pcr) assay for detection of ai. in infected animals, ai...

alfalfa crops were surveyed for the incidence of alfalfa mosaic virus (amv), cucumber mosaic virus (cmv), peanut stunt virus (psv), bean leaf roll virus (blrv), bean yellow mosaic virus (bymv) and bean common mosaic virus (bcmv) in the major growing areas in the southeast and central regions of iran. samples were collected between may 2009 and march 2011 and analyzed for viral infection initial...

b a ckground: west nile virus (wnv) is a mosquito-borne disease that can cause fatal infection in mammals in-cluding humans, dogs, horses, birds and reptiles. although west nile virus is an asymptomatic infection, especially it can cause neurologic disorders in humans and horses. the aim of this study was to the investigate virological pres- ence of wnv in horses in the black sea region of turk...

A single tube, fluorogenic probe-based, real-time quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) assay was developed for detection and quantitation of bovine respiratory syncytial virus (BRSV) using BioRad's iCycler iQ. Real-time Q-RT-PCR was compared with quantitative competitive RT-PCR (QC-RT-PCR) and viral titers. Viral mRNA levels were measured in BRSV-infected bovi...

The basis for the identification of SARS-CoV-2 cases in handling COVID-19 pandemic is Reverse Transcriptasi Polymerase (RT-PCR) and specific rapid antigen test. RT-PCR test takes a long time expensive to get results, while simple fast alternative getting detection results. method used descriptive observational form literature review by reviewing existing literature. Several screening confirmato...

Introduction: The novel coronavirus disease 2019 (COVID-19) has huge impact on public health. RT-PCR of respiratory samples is generally accepted confirmatory test which can miss several cases due to various factors. Case description: A 32-years-old male without any co-morbidity presented with complaints cough and fever was negative for Reverse Transcription Polymerase Chain Reaction (RT-PCR) t...

Serum samples from 96 Spanish hemodialysis patients, as well as serial dilutions of RNA extracted from a reference strain of hepatitis G virus (HGV), were tested for HGV or GB virus C (GBV-C) RNA. Two different reverse transcription (RT)-PCR-based methods of detection were compared for the ability to detect RNA extracted from the samples: an RT-nested PCR assay with primers derived from the 5' ...