Wolbachia is an obligatory, intracellular α-proteobacterium which infect the reproductive and somatic tissues of some arthropod and nematode populations. Because there are not any available data on the presence of this bacterium in Iran, the present study was done to determine the presence of this bacterium among 30 species of arthropods and nematodes. After DNA extraction from samples, we scre...

background: we evaluated the genetic diversity of samples identified morphologically as fasciola spp. from sheep, cattle and goat from kermanshah province, western iran using pcr-rflp method. methods: we used pcr-rflp analysis of ribosomal its1 fragment using rsai restriction enzyme to investigate the genetic characteristics of fasciola species obtained from different hosts (16 sheep, 28 cattle,...

abstract background: we aimed to describe morphological and morphometrical characteristics of fasciola spp. in livestock from ardabil province, northwest iran. methods: forty adult flukes were collected from different definitive hosts (cattle and sheep). previously specimens were identified as f. hepatica or f. gigantica based on pcr-rflp of the its-1 region with rsai enzyme.  we identified fas...

wolbachia is an obligatory, intracellular α-proteobacterium which infect the reproductive and somatic tissues of some arthropod and nematode populations. because there are not any available data on the presence of this bacterium in iran, the present study was done to determine the presence of this bacterium among 30 species of arthropods and nematodes. after dna extraction from samples, we scre...

Lemaire and Muller-Hill recently reported the nucleotide sequence of the gal T gene of Escherichia coli and deduced the amino acid sequence of galactose 1-phosphate uridylyltransferase (EC 2.7.7.12) (1). We determined the nucleotide sequence of this gene by application of the Sanger dideoxy method to EcoRV, Sau3A, RsaI and PvuII restriction fragments. Approximately 80% of the sequence was deter...

Melatonin receptor 1A gene is the prime receptor mediating the effect of melatonin at the neuroendocrine level for control of seasonal reproduction in sheep. The aims of this study were to examine the polymorphism pattern of coding sequence of MTNR1A gene in Chokla sheep, a breed of Indian arid tract and to identify new variations in relation to its aseasonal status. Genomic DNAs of 101 Chokla ...

The effect of the melatonin receptor 1a (MTNR1A) gene on fertility and litter size in autumn lambing was studied with 373 ewes from a population of sheep selected for 10 yr for fertility in May and June matings. Animals were from a composite line of 50% Dorset, 25% Rambouillet, and 25% Finnsheep. Two restriction fragment length polymorphisms were present in the population, with allelic frequenc...