Mini-cutting technique of tree species is an effective method maintaining desirable features plant matrices and uniformity. The objective the present research was to determine substrate concentration indolebutyric acid (IBA) more suitable for mini-cuttings Carya illinoinensis rooting. experiment carried out in a greenhouse with mist, from February May 2018. used experimental design blocks facto...

This study was undertaken to standardize an efficient protocol for in vitro propagation of ornamental bamboo Bambusa tuldoides Munro using nodal segments as explants. For direct shoot induction, liquid MS medium containing 2.0 mg/l BAP and 1.0 TDZ gave a maximum response (84%) with number shoots (4.80 ± 0.49) per explant. These were proliferated efficiently on agitated 3.0 1.5 mg/1 TDZ, which r...

Aconitum violaceum Jacq. ex Stapf is a threatened medicinal plant with restricted global distribution. The highest frequency of seed germination was recorded on Murashige and Skoog’s (MS) basal medium, supplemented 0.5 mg L?1 kinetin rate 77.32% mean time 27 days. Among the various growth regulators examined, 0.1 (Kn) + indole-3-acetic acid (IAA) proved to be effective for maximum embryogenic c...

A substantial number of micropropagated plants do not survive transfer from in vitro conditions to greenhouse or field environment. The greenhouse and field have substantially lower relative humidity, higher light level and septic environment that are stressful to micropropagated plants compared to in vitro conditions. The benefit of any micropropagation system can, however, only be fully reali...

To establish an efficient regeneration system for Anthurium andreanum cv Rubrun, seeds from plant spadixes were germinated on a medium supplemented with 2.2 μ M BA. After 2 weeks, 74% of the seeds germinated and four weeks later, micro-cuttings from these plantlets were subcultured on a medium containing 4.4 μ M BA and 0.05 μ M NAA. On average, 3.6 shoots per explant were obtained. Four weeks o...

We used in vitro callus and shoot cultures as target material for genetic transformation of silver birch (Betula pendula Roth) by particle bombardment. Cultivation of in vitro shoot cultures before particle bombardment and a long selection period, combined with a high concentration of selective agent after bombardment, led to the production of transformed plantlets that were stable, and no esca...

Successful in vitro propagation through ex vitro rooting mechanism has been achieved in Piper longum by nodal shoot segment cultures. Shoot tip explants were less proliferative compared to the nodal meristems. The explants were sterilized using 0.1% HgCl2 and cultured on Murashige and Skoog (MS) medium with various concentrations of growth regulators. MS medium supplemented with 1.0 mg/L 6-benz...

The paper presents protocol for rapid, reliable and high frequency regeneration of plant via the culture of leaf explants in Thevetia peruviana (Pers.) Schum. In vivo leaves cultured on MS medium supplemented with a combination of 2,4-D (2.5 mg/l) and Kn (1.2 mg/l) produced stock callus after 20-28 days of inoculation. The obtained callus was dark green and friable. The small pieces of stock ca...