The nuclear envelope separates and protects DNA from the cytoplasm. In cancer cells, frequent morphological abnormalities transient rupture of membrane promote damage malignancy. However, instability, acting as a tumor-promotion factor, can be viewed fatal weakness cells. Although exact mechanism is not clear, it known that loss tumor suppressor gene TP53 involved in instability this study, we ...

The authors have developed a method for large-scale isolation of metaphase chromosomes from HeLa cells. The distinguishing feature of this method is the use of a pH sufficiently low (about 3) to stabilize the chromosomes against mechanical damage. Many milligrams of fairly pure, morphologically intact chromosomes can be isolated in 8 hr or less of total working time. The isolated chromosomes co...

We use Förster Resonant Energy Transfer (FRET) in order to measure the increase of flexibility of short ds-DNA induced by the intercalation of dipyridophenazine (dppz) ligand in between DNA base pairs. By using a DNA double strand fluorescently labeled at its extremities, it is shown that the end-to-end length increase of DNA due to the intercalation of one dppz ligand is smaller than the DNA b...

ZC3H14 proteins bind poly (A) tail of RNAs through the CCCH (5) zinc finger domain, shuttle between the nucleus and the cytoplasm, and play roles in nuclear exporting and proper polyadenylation of mRNA transcripts. However, molecular and cellular mechanisms of mammalian ZC3H14 remain largely unknown. Here we identified the mouse Zc3h14 gene and its encoded Zc3h14 protein. We found that Mouse Zc...

Sunlight inactivation in fresh (river) water of fecal coliforms, enterococci, Escherichia coli, somatic coliphages, and F-RNA phages from waste stabilization pond (WSP) effluent was compared. Ten experiments were conducted outdoors in 300-liter chambers, held at 14C (mean river water temperature). Sunlight inactivation (k(S)) rates, as a function of cumulative global solar radiation (insolation...

Cerebral ischemia injury is a clinical, frequently occurring disease, which causes a heavy burden on society and families. It has been demonstrated that cytosolic phospholipase A2α (cPLA2α) is significant in neurological injury caused by ischemic brain injury, and inhibition of cPLA2α may reduce stroke injury. In the present study, the role of cPLA2α was investigated in a mouse model of middle ...

trichophyton rubrum is an anthropophilic fungus causing up to 90% of chronic cases of dermatophytosis. several properties of this fungus have been investigated so far. however, a few studies were carried out in the field of molecular biology of this fungus. in the present study, we tried to identify the subunit g of its vacuolar atpase (v-atpase). pairs of 21 nt primers were designed from highl...

BACKGROUND Growth arrest and DNA-damage-inducible protein 45β reactivates methylation-silenced neural plasticity-associated genes through DNA demethylation. However, growth arrest and DNA-damage-inducible protein 45β-dependent demethylation contributes to neuropathic allodynia-associated spinal plasticity remains unclear. METHODS Adult male Sprague-Dawley rats (654 out of 659) received a spin...

Root-knot nematodes (RKN; Meloidogyne incognita) are phytoparasitic that cause significant damage to crop plants worldwide. Recent studies have revealed RKNs disrupt various physiological processes in host plant cells induce gall formation. However, little is known about the molecular mechanisms of formation induced by nematodes. We previously found RNA expression levels some genes related micr...