DNA barcode is a very short, standardized DNA sequence in a well-known gene. It provides a way to identify the species to which a plant, animal or fungus it belongs. One of the most popular sequences identification is achieved by comparing DNA sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal cistron. DNA barcoding is a new concept of identifying unknown organisms b...

Ribosomal RNAs (rRNAs) in budding yeast are encoded by ~100-200 repeats of a 9.1kb sequence arranged in tandem on chromosome XII, the ribosomal DNA (rDNA) locus. Copy number of rDNA repeat units in eukaryotic cells is maintained far in excess of the requirement for ribosome biogenesis. Despite the importance of the repeats for both ribosomal and non-ribosomal functions, it is currently not know...

The genetic relatedness among various strains of halophilic bacteria has been assessed by deoxyribonucleic acid-deoxyribonucleic acid (DNA-DNA) duplex formation and ribosomal ribonucleic acid (RNA) hybridization. All of the strains of extremely halophilic rods are closely related, and the extent of divergence of base sequence is similar for the major and minor DNA components. Parallel experimen...

Ultraviolet-B (UV-B) photons can cause substantial cellular damage in biomolecules, as is well established for DNA. Because RNA has the same absorption spectrum for UV as DNA, we have investigated damage to this cellular constituent. In maize (Zea mays) leaves, UV-B radiation damages ribosomes by crosslinking cytosolic ribosomal proteins S14, L23a, and L32, and chloroplast ribosomal protein L29...

The DNA sequence of the intragenic region of the rat 45S ribosomal RNA precursor was determined. This sequence contains 2282 nucleotides and extends from the conserved EcoR I site near the 3' terminus of 18S rRNA to 69 nucleotides downstream of the 5' terminus of 28S rRNA. The sequences corresponding to 18S and 5.8S rRNA were identified by comparison with previously published data. The 5' termi...

objective: molecular epidemiology of visceral leishmaniasis (vl) is currently used widely for different objectives such as vector incrimination studies. materials and methods: in this study three different loci including kinetoplast dna (kdna), ribosomal dna (rdna), and cystein protease b (cpb) of leishmania parasite genome were used for detection and identification of natural infection of san...

Many deep-water coral species have very broad global distributions and are eurybathic from depths of meters to kilometers. Such ecological breadth may be confounded by the presence of cryptic species. We are currently comparing the genetic distances between Paragorgia sp. and Primnoa sp. across their distribution and depth range in Canada using 18S ribosomal DNA (rDNA) sequences. Initial result...

  Objective(s): Fascioliasis is a zoonotic parasitic disease caused by liver fluke species of Fasciola hepatica and Fasciola gigantica. Differentiation of these two species, based on their morphological characteristics, is difficult. The current study aimed to use PCR-RFLP assay to distinguish between F. hepatica and F. gigantica , based on profiles of RFLP, produced by effect of endo...

The genus Ononis,embraces more than 85 species worldwide. In the present study, materials of two subspecies of O. spinosa from different localities of Iran alongside some other native species of the genus were included in phylogenetic analyses. In addition, over 50 accessions were obtained from GenBank. In order to clarify the exact number of subspecies of O. spinosa in Iran, datasets were obta...