The objective of this study was to determine in vitro activities of azithromycin (AZM), clarithromycin (CLR), and 20 other antimicrobial agents against Rhodococcus equi and other common equine bacterial pathogens. A total of 201 bacterial isolates from various equine clinical samples were examined. CLR was more active than AZM against R. equi, with MICs at which 90% of the isolates were inhibit...

Rhodococcus equi per se and R. equi containing a virulence-associated plasmid can be cultured with similar frequency from the soil of farms with and without a history of R. equi disease. This finding, in conjunction with variability in results between testing procedures and laboratories, indicates that identification of R. equi by culture from farm soils does not accurately predict the probabil...

Endoglycoceramidase (EGCase) is a glycosidase capable of hydrolyzing the β -glycosidic linkage between the oligosaccharides and ceramides of glycosphingolipids (GSLs). Three molecular species of EGCase differing in specificity were found in the culture fluid of Rhodococcus equi (formerly Rhodococcus sp. M-750) and designated EGCase I, II, and III. This study describes the molecular cloning of E...

Rhodococcus equi is a facultative intracellular, Gram-positive, soilborne actinomycete which can cause severe pyogranulomatous pneumonia with abscessation in young horses (foals) and in immunocompromised people, such as persons with AIDS. All strains of R. equi isolated from foals and approximately a third isolated from humans contain a large, ~81-kb plasmid which is essential for the intramacr...

The ability of Rhodococcus rhodochrous (NCIMB 9703) to catalyse the regio- and stereoselective hydroxylation of a range of benzyloxy-substituted heterocycles has been investigated. Incubation of 2-benzyloxytetrahydropyrans with resting cell suspensions of the organism yielded predominantly a mixture of 5-hydroxylated isomers in combined yields of up to 40%. Exposure of the corresponding 2-benzy...

phenol is an environmental pollutant present in industrial wastewaters such as refineries, coal processing and petrochemicals products. in this study three phenol degrading bacteria from arak petrochemical complex effluent were isolated which consume phenol. molecular analysis was used to identify bacteria and isolated bacteria were identified as rhodococcus pyridinivorans (ns1), advenella faec...

background: the combustion of sulfur-rich fossil fuels leads to release of sulfur oxide pollution in the environment. in biodesulfurization process, an organism is able to remove sulfur from fossil fuels without decreasing the caloric value of those substrates. the main aim of this research was to design a recombinant microorganism to remove the highest amount of sulfur compounds in fossil fuel...

The prevalence of the plasmid-encoded virulence-associated gene (vapA) of Rhodococcus equi, as determined by PCR, was found to be 98% in isolates from 154 foals with pneumonia, confirming the strong association of vapA with virulence. The vapA genes from 60 representative isolates were compared by digestion with the restriction endonuclease HinfI, and no evidence of sequence variation was detec...

Two-dimensional ridge structures are induced by equi-biaxial compression with large equi-biaxial pre-stretch in a thin film by using a micro-fluidics technique. Whereas wrinkles tend to be uniformly distributed, ridges are localized. The wrinkle-to-ridge transition is unstable (subcritical), resulting in large amplitude changes. The nature and morphology of the ridges is studied and quantified ...

Fifty-four strains of Rhodococcus equi from different clinical sources (mainly horses and pigs) were examined for their plasmid content by two screening methods. Plasmids were detected in 49 of 54 strains. A plasmid of approximately 80 kb was isolated from 21 of 22 isolates from horses and 20 of 28 isolates from pigs, and a 105-kb plasmid was isolated from 7 of 28 isolates from pigs. The 80-kb ...