In Pseudomonas aeruginosa, azithromycin has been shown to reduce virulence factor production, to retard biofilm formation, and to exhibit bactericidal effects on stationary-phase cells. In this study we analyzed whether these azithromycin-mediated effects require interaction with the ribosome. We blocked the access of azithromycin to the ribosome in P. aeruginosa PAO1 by expressing the 23S rRNA...

In this paper, a thorough experimental investigation of enhanced oil recovery via controlled salinity-biosurfactant injection under typical reservoir temperature conditions is reported for the first time. Sixteen core flooding experiments were carried out with four displacing fluids in carbonate rock samples and improved was investigated secondary, tertiary quaternary modes. The effect on durin...

Metabolic engineering of microbial cell factories for the production of heterologous secondary metabolites implicitly relies on the intensification of intracellular flux directed toward the product of choice. Apart from reactions following peripheral pathways, enzymes of the central carbon metabolism are usually targeted for the enhancement of precursor supply. In Pseudomonas putida, a Gram-neg...

The current study evaluated hydrocarbon-degrading and biosurfactant-producing potentials of bacteria isolated from hydrocarbon contaminated soil in Nigeria, the largest crude oil reservoir Africa. Pure bacterial isolates were grown on nutrient Bushnell Haas agar. Bacterial that grew both media molecularly identified via 16S rDNA sequence. Biosurfactant production detection was carried out sprea...

Biosurfactants are a microbially synthesized alternative to synthetic surfactants, one of the most important bulk chemicals. Some yeast species proven be exceptional biosurfactant producers, while others emerging producers. A set factors affects type, amount, and properties produced, as well environmental impact costs biosurfactant’s production. Exploring waste cooking oil substrate for biosurf...