The aims of the present study were to propose apolymerase chain reaction-restriction fragment length polymorphismPCR-RFLP genotyping method for the AY_376689:c.773C>T single nucleotide polymorphism SNP of the equine PRKAG3 gene, related to muscle performance, as well as to characterize this SNP and a second polymorphism, AAWR_02017454:g.121684T>C of the SPATA1 gene, related to male fertility, i...

Present investigation was carried out to identify DNA polymorphism of IL-2Rγ and ChB6 genes. Sixty five birds belonging to the indigenous chicken of Himachal Pradesh were utilized. Good quality DNA samples were subjected to PCR-RFLP analysis using chicken specific primers. Overnight restriction enzyme digestion was carried out at 37°C with IU Hph I and Pvu II for IL-2Rγ and ChB6 genes, respecti...

STAT5 is a group of transcription factors that mediate signals from prolactin and growth hormone. Therefore, STAT5A gene is a candidate marker for quantitative traits in farm animals. In this study the new nucleotide sequence polymorphism was found in the coding region of the bovine STAT5A gene substitution C→T at position 6853 within the exon 7. As this mutation creates new AvaI/DdeI restricti...

Leptin, a peptide hormone secreted by adipose tissue cells, has been implicated in regulation of feed intake, energy balance, fertility, immune functions, and the neuroendocrine axis in rodents, humans and large domestic animals. The objective of present study was to determine the polymorphic patterns of leptin gene in Iranian native cattle using the PCR-RFLP technique. Bull semen specimens wer...

The gene encoding the MT1 melatonin receptor in sheep has a restriction fragment length polymorphism (RFLP) site to the MnlI enzyme whose incidence is associated to the expression of seasonality in several breeds. The aim of this study was to examine the relationship between this genetic marker and the physiological effects of MT1 receptor gene polymorphism on several seasonal functions in Ile-...

The Rice TOGO Browser is an online public resource designed to facilitate integration and visualization of mapping data of bacterial artificial chromosome (BAC)/P1-derived artificial chromosome (PAC) clones, genes, restriction fragment length polymorphism (RFLP)/simple sequence repeat (SSR) markers and phenotype data represented as quantitative trait loci (QTLs) onto the genome sequence, and to...

The cladocerans are important components of planktonic and benthic freshwater and good indicators of the trophic state of water bodies. The morphological taxonomy of many species of Cladocera is considered complex with minor differences separating some species. Nowadays, molecular techniques provide a powerful tool to identify and classify different taxonomical levels, using mainly ribosomal RN...

Populations of Rhizobium leguminosarum biovar viciae were sampled from two bulk soils, rhizosphere, and nodules of host legumes, fava bean (Vicia faba) and pea (Pisum sativum) grown in the same soils. Additional populations nodulating peas, fava beans, and vetches (Vicia sativa) grown in other soils and fava bean-nodulating strains from various geographic sites were also analyzed. The rhizobia ...

the objective of the present study was to detect polymorphism in follicle stimulating hormone receptor (fshr) and beta subunit of follicle stimulating hormone (fshβ) genes and their relation to litter size and body weight trait in baluchi, iran black and arman sheep breeds. pcr-rflp technique using acci and hinfi enzyme treatments employed to detection of polymorphism in fshβ marker site but de...

Toxoplasma gondii is the causative protozoan agent of toxoplasmosis, which is a common infection that is widely distributed worldwide. Studies revealed stronger clonal strains in North America and Europe and genetic diversity in South American strains. Our study aimed to differentiate the pathogenicity and sulfadiazine resistance of three T. gondii isolates obtained from livestock intended for ...