Background and objectives: The rhizomes of Eremostachys azerbaijanica Rech. f.,as an indigenous plant in East Azerbaijan province of Iran, were studied for isolation and identification of possible phytoconstituents. Methods: The air- dried and ground rhizomes were extracted with n-hexane, dichloromethane (DCM) and methanol (MeOH) using a Soxhlet appara...

Background and objectives: Colocynthin is the major active secondary metabolite of colocynth, Citrullus colocynthis (L.) Schrad, which has been used in traditional and ethno medicine of many countries.  It could be considered as an active marker for quality control of colocynth and its herbal products. Analysis and standardization of colocynth and its herbal preparations are a critical...

Sensitive and selective liquid chromatography-tandem mass spectrometry methods have been developed and validated for the determination of delamanid in mouse plasma and lung. Sample preparation involved liquid-liquid extraction with diethyl ether for plasma, or protein precipitation followed by liquid-liquid extraction with tert-butyl methyl ether for lung homogenate. Chromatographic separation ...

Acalypha indica, a known medicinal plant in the treatment of various degenerative diseases and a potential source of valuable bioactives. The present study investigates the total phenolic and flavonoid content and also screen for phytocompounds in A. indica leaf extract obtained by two extraction methods, comparatively. Results indicated that the maceration extraction method (MEM) had highest t...

In previous studies, mebudipine, a dihydropyridine calcium channel blocker, showed a considerable potential to be used in cardiovascular diseases. The aim of the current study was to develop a valid method using reversed-phase high performance liquid chromatography coupled with electrospray ionization mass spectrometry to assay mebudipine in the human plasma. Separation was achieved on a Zorbax...

Mefloquine, as a racemic mixture, is used for the treatment and prophylaxis of malaria. Stereoselective pharmacodynamic and pharmacokinetic differences has been observed for mefloquine. In this study a modified stereoselective HPLC method is presented for determination of mefloquine (MFQ) enantiomers in whole blood. The assay involved liquid-liquid extraction of MFQ from biological fluids with ...

This research paper presents findings of an investigation about antibacterial effect of methanol/water fractions as well as cytotoxic activity of the extracts obtained from Pedicularis wilhelmsiana (Scrophulariaceae) which grows in Azarbaijan/Iran by agar well diffusion method and brine shrimp lethality test successively. Phytochemical study of this plant was determined as well. A combination o...

A rapid, new analytical method, based on liquid chromatography with UV detection, has been developed and utilised for the determination of biperiden hydrochloride in solid dosage forms. The chromatographic separation was performed a conventional C18 reversed phase column mobile composed acetonitrile-buffer mixture. Ultraviolet detection carried out at 205 nm. rapid extraction procedure using ul...