نتایج جستجو برای: reverse transcriptase polymerase chain reaction
تعداد نتایج: 757054 فیلتر نتایج به سال:
The application of membrane technology in water and wastewater treatment is increasing due to stringent water quality standards. Nanofiltration (NF) is one of the widely used membrane processes for water and wastewater treatment in addition to other applications such as desalination. NF has replaced reverse osmosis (RO) membranes in many applications due to lower energy consumption and higher f...
Background and Aims: Influenza virus is a major pathogen involved in respiratory illnesses during winter seasons. A variety of diagnostic methods have been developed to identify influenza viruses in clinical specimen. Methods: Nasal and pharyngeal samples taken from patients were inoculated into Madin-Darby canine kidney (MOCK) cells and embryonated chicken eggs (ECEs). The culture media was a...
Background and Aims: The aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcDNA3.1(+) in BSR cell line. This construct might be used for a potential DNA vaccine. Materials and Methods: Glycoprotein gene was synthesized and cloned into pBluescript vector and then sub cloned into eukaryotic expression vector (pcDNA3.1(+)). After verifica...
Reaction times (RTs) from human orofacial muscles were obtained with labial displacement and auditory stimuli to evaluate the potential for contributions by these sensorimotor processes to speech movement coordination. Because speech requires both concurrent and sequential movements, intramovement (labial stimulus, labial response) and intermovement (labial stimulus, lingual response) RT minima...
The highly pathogenic Marburg virus (MARV) is a member of the Filoviridae family and belongs to the group of nonsegmented negative-strand RNA viruses. Reverse genetics systems established for MARV have been used to study various aspects of the viral replication cycle, analyze host responses, image viral infection, and screen for antivirals. This article provides an overview of the currently est...
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. pISSN 1011-8934 eISSN 1598-6357 http://dx.doi.org/10.3346/jkms.2011.26.10.1399 • J Korean Me...
HIV-1 replication is inhibited by the incorporation of chain-terminating nucleotides at the 3' end of the growing DNA chain. Here we show a nucleotide-dependent reaction catalyzed by HIV-1 reverse transcriptase that can efficiently remove the chain-terminating residue, yielding an extendible primer terminus. Radioactively labeled 3'-terminal residue from the primer can be transferred into a pro...
The RT-CaCCO process for enzymatic saccharification was applied to readily-milled fractions of wet sugarcane bagasse. Wet bagasse immediately after juice extraction was crushed with shark-mill blades to prepare two fractions referred to as readily-milled (RF) and hardly-milled fraction (HF). Monosaccharide recoveries from RFs via the RT-CaCCO process were 1.03-1.21 times higher than those from ...
........................................................................................................................................... IX 1. GENERAL INTRODUCTION .......................................................................................................... 1 2. LITERATURE REVIEW .......................................................................................................
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