Adverse effects of strain persistence and secretion in milk have been encountered with the Brucella melitensis vaccine strain Rev.1. Field isolates obtained from vaccinated animals and from a human resembled the vaccine strain Rev.1 by conventional bacteriological tests. The lack of a specific molecular marker that could specifically characterize the commercial vaccine strain prevented confirma...

Expression of the structural proteins of human immunodeficiency virus type 1 requires the direct interaction of multiple copies of the viral Rev protein with its highly structured RNA target sequence, the Rev response element (RRE). Nucleotides critical for Rev monomer binding have been mapped by chemical interference to a single site flanking the base of an RNA helix (stem IIB) located within ...

the aim of this study was to prepare liposomes of acyclovir (acy) by thin layer evaporation (tle) and reverse phase evaporation (rev) methods. twenty-seven batches of liposomes from each method were prepared using technique of three variables at three levels (33) factorial design. drug/lipid (molar ratio), hydration volume and hydration time were considered three independent variables in tle me...

and Julieke Hupkes (unpublished). P. A. Flinn (private communication). R. W. Grant, R. M. Housley, and U. Gonser, Phys. Rev. 178, 523 (1969). ~~S. Goldsztaub, Bull. Soc. Franc. Mineral 58, 6 (1935). R. M. Housley, N. E. Erickson, and J. G. Dash, Nucl. Instr. Methods 27, 29 (1964). An additional small background correction of about 2/o was made to account for Compton scattering from a Lucite sou...

The human immunodeficiency virus type-1 Rev protein induces the nuclear export of intron-containing viral mRNAs that harbor its binding site, the Rev response element (RRE). A leucine-rich region of Rev, the activation domain, is essential for function and has been shown to be a nuclear export signal (NES). Although Rev exports viral RNAs that resemble cellular mRNAs, competition studies perfor...

A single transcript in its unspliced and spliced forms directs the synthesis of all HIV-1 proteins. Although nuclear export of intron-containing cellular transcripts is restricted in mammalian cells, HIV-1 has evolved the viral Rev protein to overcome this restriction for viral transcripts. Previously, CRM1 was identified as a cellular cofactor for Rev-dependent export of intron-containing HIV-...

Gene expression of the human immunodeficiency virus type 1 (HIV-1) is a highly regulated process. Basal transcription of the integrated provirus generates early transcripts that encode for the viral products Tat and Rev. Tat promotes the elongation of RNA polymerase while Rev mediates the nuclear export of viral RNAs that contain the Rev-responsive RNA element (RRE). These RNAs are exported fro...