Bronchoscopes and the filters used for washing them were found to yield high numbers of Mycobacterium avium isolates sharing the same repetitive sequence-based PCR (rep-PCR) fingerprint pattern as M. avium isolates recovered from patient samples collected by bronchoscopy. Water and biofilm samples collected from the bronchoscopy preparation laboratory yielded M. avium, Mycobacterium intracellul...

The applicability of the repetitive-sequence-based PCR (rep-PCR)–based DiversiLab system was tested compared with the pulsed fieldgel electrophoresis (PFGE) to type a phenotypically similar subset of a large collection of multiresistant Pseudomonas aeruginosa strainsisolated during a 17-month period from patients treated in different wards including 4 intensive care units (ICUs). Five e...

Tuberculosis remains a severe public health problem worldwide. Presently, genotyping is used for conducting epidemiologic and clinical studies on tuberculosis cases. We evaluated the efficacy of the repetitive sequence-based PCR (rep-PCR)-based DiversiLab™ system (bioMérieux, France) over the IS6110-restriction fragment length polymorphism analysis for detecting Mycobacterium tuberculosis. In a...

In the present study, out of 264 phosphate (P) solubilizing Bacillus strains isolated from apple rhizosphere, only twelve isolates were found to be efficient (showed most of the plant growth promoting activity) which were further characterized at molecular level using 16S rDNA partial gene sequencing. Out of 12 isolates, MZPSB 207 was found to be most efficient P-solubilizing (864.71 μg/ml) iso...

Bacterial cellulose (BC) is receiving a great deal of attention due to its unique properties such as high purity, water retention capacity, mechanical strength, and biocompatibility. However, the production BC has been limited because associated costs low productivity. In light this, isolation new producing bacteria selection highly productive strains become prominent issue. Kombucha tea fermen...

yersinia enterocolitica is rapidly emerging worldwide as an enteric pathogen and has become a major cause of diarrhea even in developed countries. the aim of this study was to characterize and genetic diversity analysis among y. enterocolitica strains isolated from fish and chicken sources.from 44 strains, 55% (24 strains) found to be positive for y. enterocolitica by colony morphology, biochem...

anthracnose caused by colletotrichum gloeosporioides s. l. is one of the most important diseases of citrus in northern iran. to study the genetic structure of c. gloeosporioides s. l. from citrusspp., infected samples were collected from three citrus cultivating regions of northern iran, during the summer of 2009. fifty one monoconidial isolates were used as the objective of rep- and box-pcr fi...

Lactobacilli were isolated from jejunal chyme from five fistulated beagles. Cultivable lactobacilli varied from 10(4) to 10(8) CFU/ml. Seventy-four isolates were identified by partial 16S rRNA gene sequencing and differentiated by repetitive element PCR (Rep-PCR), Lactobacillus acidophilus was dominant, and nearly 80% of 54 isolates shared the same DNA fingerprint pattern.