Protoplasts (2 x 10(7)/g fresh wt) were isolated from leaves of A. thaliana ecotype estland, with a viability of more than 90%. Protoplasts cultured in calcium alginate beads or layers showed division while culture in liquid or agarose beads failed to elicit any division. Effect of culture density showed highest frequency of division occurring at 5 x 10(5) while no division was seen when cultur...

An efficient plant regeneration protocol has been developed from leaf explants of Stevia rebaudiana, an economically and medicinally important herbaceous plant species belonging to the family Asteraceae. Plant regeneration and multiple shoot proliferation was initiated from leaf explants cultured on MS medium supplemented with various concentrations of 6-benzyladenine (BA) and kinetin (KN) in c...

Procedures for forming and regenerating protoplasts of four Frankia strains are described. Cells obtained from growth medium containing 0.1% glycine were digested with lysozyme (250 mug/ml) in a medium containing 0.5 M sucrose, 5.0 mM CaCl(2), and 5.0 mM MgCl(2). Protoplasts were formed during 15 to 120 min of digestion at 25 degrees C. Optimum conditions for protoplast regeneration involved pl...

A simple and efficient plantlet regeneration protocol via direct organogenesis was established for camphor tree (Cinnamomum camphora L.). Stem segments with one node (SN explants) from embryo-cultured seedlings (EC seedlings) were used as explants. Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2, 4-dichlorophenoxyacetic acid and 2.0 mg/L 6-benzyladenine was used to induce cotyledon...

Different concentrations of N(6)-benzylaminopurine (BAP) and indole acetic acid (IAA) in Murashige and Skoog based medium were assessed for their effects on shoot multiplication, nodule-like meristem proliferation and plant regeneration of the Malaysian banana cultivars Pisang Mas, Pisang Nangka, Pisang Berangan and Pisang Awak. BAP at 1-14 mg L(-1) with or without 0.2 mg L(-1) IAA, or BAP at 7...

We study J/psi production at RHIC and LHC energies with both initial production at energies reached and the BNL Relativistic Heavy Ion Collider (RHIC) and CERN Large Hadron Collider (LHC) with regeneration. We solve the coupled set of transport equations for the J/psi distribution in phase space and the hydrodynamic equation for evolution of quark-gluon plasma. At RHIC, continuous regeneration ...

In five cultivated rice varieties e.g., Nonabokra, PNR 381, Taipei 309, Koshika and TKM 11 the response in the initiation of callus and its size varied greatly among varieties, salt concentrations (NaCl and Na2SO4) and their interaction. Better regeneration ability of Nonabokra, Koshika and TKM 11 was found to be correlated to small size callus with a high concentration of Na2SO4. The regenerat...

The success of plant tissue cultures is influenced by plant growth regulators, nutrient supply, and the carbon source in the medium. We investigated the most suitable carbon source and concentration for the in vitro rooting of Rehmannia glutinosa. Stem segments of R. glutinosa were cultured in vitro with 30 g/L of seven different carbon sources (sucrose, dextrose, mannose, glucose, sucrose, gal...