نتایج جستجو برای: recombinant egb82 protein

تعداد نتایج: 1297257  

Journal: : 2023

Synthesis of Trimethyl Chitosan Nanoparticles Containing Recombinant BLF1-stxB Protein Burkholderia Pseudomallei and Evaluation its Immunogenicity in Mice

ژورنال: Medical Laboratory Journal 2015
Bakhshi , Parisa , Mirzahoseini, Hassan , Mohajerani, Nazanin , Saidijam, Massoud , Shahbazzadeh, Delavar ,

Abstract         Background and Objective: Hirudin is an anticoagulant polypeptide secreted from the salivary glands of leeches. Recombinant hirudin is a strong anticoagulant agent in arterial and venous thrombosis. The aim of this study was to evaluate the effect of inserting protein A signal peptide sequence of pEZZ18 plasmid on expression and secretion...

Journal: :iranian journal of biotechnology 2008
malihe nazari jahantigh kamran ghaedi mohamad hossein nasr isfahani somayeh tanhaei farzaneh rabiee

epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant dna techniques. the aim of this study was to sub-clone the peroxisomal protein (pep) cdna into a mammalian expression vector leading to the formation of a  chimeric pep-cdna containing the flag epitope. the flag-pep recombinant cdna was construc...

Journal: :iranian journal of microbiology 0
hamid sedighian rad applied microbiology research center, baqiyatallah medical science university, tehran. seyed-latif mousavi department of biology, faculty of basic sciences, shahed university, tehran, iran. iraj rasooli department of biology, faculty of basic sciences, shahed university, tehran, iran. jafar amani applied microbiology research center, baqiyatallah medical science university, tehran. moohamad-reza jalali-nadooshan department of pathology, faculty of medical sciences, shahed university, tehran, iran.

background and objective: enterohemorrhagic escherichia coli (ehec) o157:h7 is an important enteric pathogen in human causing bloody or nonbloody diarrhea, which may be complicated by hemolytic uremic syndrome (hus). cattle are an important reservoir of ehec. this research aims at vaccination with a divalent chimer protein composed of espa 120 and intimin 282 and its preventive effect of ehec o...

Journal: :jundishapur journal of microbiology 0
fatemeh abedi jafari molecular biology department, pasteur institute of iran, tehran, ir iran mana oloomi molecular biology department, pasteur institute of iran, tehran, ir iran; molecular biology department, pasteur institute of iran, tehran, ir iran. tel: +98-2166953311, fax: +98-2166492619 saeid bouzari molecular biology department, pasteur institute of iran, tehran, ir iran

conclusions these results suggest that toxins induce inflammatory responses, particularly through expression of chemokine. recombinant stx and native toxin induced apoptosis by balancing between different pro- and anti-apoptotic bcl-2 family-factors in epithelial cells. in this study, for the first time, recombinant and native stx induction of apoptotic factors and stimulation of immune respons...

آرش‌کیا, آرش , رحیمی, آرین , رحیمی, رقیه , شاندیز, سیدعطا‌اله سادات , مهدوی, مهدی , میرزایی, امیر , نوربازرگان, حسن ,

Background: Human papilloma virus is a DNA virus from the papillomavirus family that is most prevalent in human cervical cancers and many studies showed the E6 and E7 proteins are present in the majority of cervical cancer cases. Development of universal HPV peptide-based vaccine with more serotypes coverage has considerable value. The aim of the study was to design a multi-epitope universal va...

Journal: :Antibody reports 2022

The recombinant antibodies RB702, RB703, RB704 and RB705 detect by ELISA a synthetic peptide from the ROR1 protein.

ژورنال: Vaccine Research 2017

Introduction: Hepatitis E virus (HEV) is a fecal-oral transmitting virus which causes a chronic liver disease. ORF2 is an immunogen capsid protein of HEV that has been proposed to be used for Hepatitis E vaccine design. It is a 660-amino acid protein which includes an immunogenic region (residues 112-607). This protein has been expressed in complete and truncated forms, using different expressi...

Hamid Madanchi Hossein Honari, Mahdi Hesaraki Mohammad Sadraeian

        Ricin, the toxic lectin extracted from the castor bean plant (Ricinus communis), consists of an A chain (RTA) and a B chain (RTB). Anti-A chain Abs and anti-B chain Abs can neutralize toxins in vivo and in vitro via blocking the binding of the toxin to the cell. Also, RTB protein is able to serve as an antigen deliver to the mucosal immune system and act as an immunoad...

Journal: Vaccine Research 2014
Behrouz Vaziri, Navid D. Djadid, Sedigheh Mirkazemi, Sedigheh Zakeri, Vahideh Valizadeh,

  Background: The purity and correct folding of a recombinant protein is critical for any structural, biochemical and vaccine design studies. Plasmodium vivax Duffy binding protein-II is a leading vaccine candidate for vivax malaria. In the present study, the purification process of recombinant DBP-IX (a variant form of PvDBP-II) was optimized to achieve the highest yield and purity. Moreover, ...

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