نتایج جستجو برای: recombinant antigens

تعداد نتایج: 218562  

Journal: :Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2004
N S Sato T Suzuki T Ueda K Watanabe R D C Hirata M H Hirata

Three recombinant antigens of Treponema pallidum Nichols strain were fused with GST, cloned and expressed in Escherichia coli, resulting in high levels of GST-rTp47 and GST-rTp17 expression, and supplementation with arginine tRNA for the AGR codon was needed to obtain GST-rTp15 overexpression. Purified fusion protein yields were 1.9, 1.7 and 5.3 mg/l of cell culture for GST-rTp47, GST-rTp17 and...

Journal: :Clinical and vaccine immunology : CVI 2009
Maja Neiman Carl Hamsten Jochen M Schwenk Göran Bölske Anja Persson

A recombinant antigen cocktail enzyme-linked immunosorbent assay (ELISA) for diagnosis of contagious bovine pleuropneumonia (CBPP) was developed after careful selection of antigens among one-third of the surface proteome proteins of the infectious agent Mycoplasma mycoides subsp. mycoides small colony (M. mycoides SC). First, a miniaturized and parallelized assay system employing antigen suspen...

2012
Ana Hernández-González Saúl Santivañez Héctor H. García Silvia Rodríguez Santiago Muñoz Guillermo Ramos Antonio Orduña Mar Siles-Lucas

A standardized test for the serodiagnosis of human cystic echinococcosis (CE) is still needed, because of the low specificity and sensitivity of the currently available commercial tools and the lack of proper evaluation of the existing recombinant antigens. In a previous work, we defined the new ELISA-B2t diagnostic tool for the detection of specific IgGs in CE patients, which showed high sensi...

Journal: :Journal of clinical microbiology 2003
Elisa Beghetto Wilma Buffolano Andrea Spadoni Mariassunta Del Pezzo Manlio Di Cristina Olga Minenkova Eskild Petersen Franco Felici Nicola Gargano

The objective of this work was to develop an antibody-specific immunoglobulin G (IgG) avidity assay to discriminate between acute and latent phases of Toxoplasma gondii infection by using recombinant antigens. One hundred twenty-one serum samples from women who developed IgG antibodies against Toxoplasma during pregnancy were used. The IgG avidities of antibodies directed against epitopes carri...

Journal: :Infection and immunity 1998
L Kremer L Dupré G Riveau A Capron C Locht

A major goal of current vaccine development is the induction of strong immune responses against protective antigens delivered by mucosal routes. One of the most promising approaches in that respect relies on the use of live recombinant vaccine carriers. In this study, Mycobacterium bovis BCG was engineered to produce an intracellular glutathione S-transferase from Schistosoma haematobium (Sh28G...

Journal: :Journal of clinical microbiology 2003
Wesley C Van Voorhis Lynn K Barrett Sheila A Lukehart Bruno Schmidt Martin Schriefer Caroline E Cameron

Syphilis serodiagnosis relies on a combination of nonspecific screening tests (antilipoidal antibodies) and Treponema pallidum-specific tests (anti-T. pallidum antibodies). We studied a group of six recombinant T. pallidum antigens for their sensitivities and specificities with sera from individuals with syphilis (n = 43), relapsing fever (n = 8), Lyme disease (n = 8), and leptospirosis (n = 9)...

Journal: :Journal of clinical microbiology 2004
H Pietkiewicz E Hiszczyńska-Sawicka J Kur E Petersen H V Nielsen M Stankiewicz I Andrzejewska P Myjak

Toxoplasma gondii SAG1, GRA1, and GRA7 recombinant antigens may be regarded as tools for the detection of T. gondii immunoglobulin G antibodies in persons with chronic and acute toxoplasmosis. GRA7 is more correlated with acute toxoplasmosis. A combination of these antigens will increase the sensitivity of enzyme-linked immunosorbent assays.

Journal: :Clinical and vaccine immunology : CVI 2007
Flavia B Dos Santos Rita Maria R Nogueira Monique R Q Lima Thatiane S De Simone Hermann G Schatzmayr Elezer M B Lemes Eva Harris Marize P Miagostovich

We have developed an indirect enzyme-linked immunosorbent assay for detection of anti-dengue virus (DENV) immunoglobulin G antibodies using four recombinant DENV envelope polypeptides as antigens, which demonstrated a sensitivity of 89.4% and a specificity of 93.3%. These easily produced antigens are a feasible, cost-effective alternative for generating reagents for dengue serological tests.

2010
Rajib Deb P. P. Goswami

Mycobacterium avium subsp. paratuberculosis (Map) contains PPE family antigens which are Proline and glutamic acid rich and may play important role as T cell antigens. Hence the identification and generation of antigens are necessary for immunological characterization. In the present study, the epitopic region of a unique PPE gene encoding 34.9 kDa protein from Map was amplified by polymerase c...

Journal: :The Korean journal of parasitology 2005
Jung-Hwa Cho Woo-Suk Chung Kyoung-Ju Song Byoung-Kuk Na Seung-Won Kang Chul-Yong Song Tong-Soo Kim

Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against N. caninum infection was evaluated in vitro and in vivo. Two major immunodominant surface antigens (NcSAG1 and NcSRS2) and two dense granule proteins (NcDG1 and NcDG2) of N. caninum tachyzoites were expressed in E. coli, respectively. An in vitro neutralization assay using polyclonal antisera raised a...

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