In the preceding accompanying paper [Shu, X., et al. (2007) Biochemistry 46, 12005-12013], the 1.5 A resolution crystal structure of green fluorescent protein (GFP) variant S65T/H148D is presented, and the possible consequences of an unusual short hydrogen bond (<or=2.4 A) between the carboxyl oxygen of Asp148 and the phenol oxygen of the chromophore are discussed. This work reports the femtose...

a simple method has been employed to synthesize copper and copper oxide nanoparticles from copper (ii) succinate precursor by thermal decomposition method using oleylamine as a capping agent. the particles synthesized with oleylamine and triphenylphosphine were well dispersed nanoparticles obtained from x-ray diffraction (xrd). the synthesized copper nanoparticles were characterized by uv-visib...

We analyze observations obtained with the Chandra X-ray Observatory of bright Compton thick active galactic nuclei (AGNs), those with column densities in excess of 1.5 × 10 cm along the lines of sight. We therefore view the powerful central engines only indirectly, even at X-ray energies. Using high spatial resolution and considering only galaxies that do not contain circumnuclear starbursts, w...

We have measured angular-dependent fluorescence-yield x-ray magnetic circular dichroism spectra on single crystals of the heterometallic $3d\ensuremath{-}4f$ 12-metallacrown-4 ${\mathrm{TbMn}}_{4}$ and ${\mathrm{DyMn}}_{4}$ complexes. Simulated using crystal-field multiplet calculations reproduce experimentally observed spectra. The orientation molecules causes linear $4f$ absorption This natur...

BACKGROUND AND OBJECTIVE Fluorescence spectroscopy has been shown to provide information useful in the detection of cervical dysplasia. The goal of this study was to determine if substances found on the cervix such as acetic acid, mucus, and vaginal medications can influence the fluorescence in the spectral region useful for discriminating normal cervical tissue from abnormal tissue. STUDY DE...

Selenium compounds exhibit chemopreventative properties at supranutritional doses, but the efficacy of selenium supplementation in cancer prevention is dependent on the chemical speciation of the selenium supplement and its metabolites. The uptake, speciation, and distribution of the common selenoamino acid supplements, selenomethionine (SeMet) and Se-methylselenocysteine (MeSeCys), in A549 hum...