We investigate a immunoassay biosensor that employs a Quartz Crystal Microbalance (QCM) to detect the specific binding reaction of the (Human IgG1)-(Anti-Human IgG1) protein pair under physiological conditions. In addition to experiments, a three dimensional time domain finite element method (FEM) was used to perform simulations for the biomolecular binding reaction in microfluidic channels. In...

The ultimate goal in any biosensor development project is its use for actual sample detection. Recently, there has been an interest in biosensors with aptamers as bio-recognition elements, but reported examples all deal with standards, not human serum. In order to verify the differences of aptamer-based biosensor and antibody-based biosensor in clinical detection, a comparison of the performanc...

The use of a quartz crystal microbalance (QCM) for monitorin g in situ the immobilization of singlestrand DNA marked with mercaptol group at the 5'-end on the surface of a gold-filled 7.995 MHz AT-cut quartz crystal by Au–S bond with the self-assembly technique is reported. The hybridization of ssDNA with complementary 10-mer ODN and 8-mer ODN is described. The QCM was also employed to analyze ...

Quartz crystal microbalance (QCM) has been extensively applied in real-time and label-free biomolecular interaction studies. However, the sensitive detection by QCM technology remains challenging, mainly due to the limited surface immobilization capacity. Here, a three-dimensional (3D) carboxymethyl dextran coated gold sensor chip surface was successfully fabricated with dextran of different mo...