Degenerate splice site sequences mark the intron boundaries of pre-mRNA transcripts in multicellular eukaryotes. The essential pre-mRNA splicing factor U2AF(65) is faced with the paradoxical tasks of accurately targeting polypyrimidine (Py) tracts preceding 3' splice sites while adapting to both cytidine and uridine nucleotides with nearly equivalent frequencies. To understand how U2AF(65) reco...

This study aimed to investigate the proapoptotic effects and the mechanism of action of pingyangmycin (PY) on cavernous hemangioma. The rat spleen was used as a model of cavernous hemangioma. PY was injected into the spleen and the pathological changes were observed at different time-points. Apoptosis was detected using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay ...

An innovative approach based on the combination of analytical pyrolysis coupled with gas chromatography-mass spectrometry (Py-GC/MS) compound-specific isotope analysis (Py-CSIA) is used to study composting process maize biomass. This multidisciplinary aims elucidate decomposition rate main biogenic materials (lignin, cellulose, proteins, lipids, and waxes) responses process. According Py-GC/MS ...

We investigate the coupled magnetization dynamics in heterostructures of a single crystal chiral magnet [Formula: see text] (CSO) and polycrystalline ferromagnet (Py) thin film using broadband ferromagnetic resonance (FMR) at cryogenic temperatures. observe excitation hybrid mode (HM) below helimagnetic transition temperature CSO. This HM is attributed to spin CSO/Py interface. study by measuri...

background treatment of helicobacter pylori (h. pylori) as a major cause of gastric diseases is of utmost concern.we aimed to assess efficacy of triple therapy (amoxicillin, clarithromycin and pantoprazole) plus probiotic yogurt (py) on eradication of h.pylori. methods total 102 h. pylori positive patients were divided to 3 groups equally and randomly. for treatment of each group amoxicillin, c...

This study presents estimates of incidence of leprosy among the familial contacts (FC) and non-familial contacts (NFC) of leprosy patients in Agra, a district endemic for leprosy. The study covers 42,113 persons followed up for 123,951.2 person years (PY) during which 77 individuals developed leprosy giving an incidence of 6.2/10,000 PY in the total leprosy-free population studied (TLPS). The i...

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants present in air and food. Among PAHs, benzo(a)pyrene(BaP), phenanthrene (PH) and pyrene (PY) are considered to be important for their toxicity or abundance. To investigate the changes of biomarkers after PAH exposure, rats were treated with BaP (150 microg/kg) alone or with PH (4,300 microg/kg) and PY (2,700 microg...

Water-dispersible and fluorescent core−shell nanorods (NRs) consisting of covalently linked poly(allyamine hydrochloride)-pyrene (PAH-Py) were synthesized and used for fabricating hybrid organic−inorganic NRs. The reaction between 1-pyrenecarboxaldehyde (Py-CHO) nanorods and PAH was driven by Schiff base formation and followed by NaBH4 reduction, and caused 28% mass loss of Py molecules at the ...

Post-translational protein modifications, such as tyrosine phosphorylation, regulate protein-protein interactions (PPIs) critical for signal processing and cellular phenotypes. We extended an established yeast two-hybrid system employing human protein kinases for the analyses of phospho-tyrosine (pY)-dependent PPIs in a direct experimental, large-scale approach. We identified 292 mostly novel p...