By shedding light on variation in time as well as in space, long-term biogeographic studies can help us define organisms' distribution patterns and understand their underlying drivers. Here we examine distributions of Pseudomonas in and around 15 human homes, focusing on the P. putida and P. fluorescens species groups. We describe recovery from 10,941 samples collected during up to 8 visits per...

Anthranilate synthase in Pseudomonas putida is a two component enzyme system. The proteins, termed AS I and AS II, have respective molecular weights of 65,000 and 18,000. Five additional Pseudomonas species, both tryptophan requiring and independent strains, were examined and all were shown to contain similar two component systems. Anthranilate formation by "amide transfer," with L-glutamine as...

Liquid chromatography/electrospray ionization/mass spectrometry (LC/ESI/MS) was used to determine intact phospholipid profiles for five reference pseudomonad strains harboring different (aerobic) toluene catabolic pathways: Pseudomonas putida mt-2, Pseudomonas putida F1, Burkholderia cepacia G4, Burkholderia pickettii PKO1, and Pseudomonas mendocina KR1. These five strains contained a predomina...