The effects on platelet aggregation of native rat chyle chylomicrons, chylomicron remnants, and chylomicrons that had been preincubated with rat or human EDTA-plasma, serum, whole blood, or pure human prothrombin were examined. The native chyle chylomicrons did not induce platelet aggregation but decreased ADP- and thrombin-induced platelet aggregation and [14C]serotonin release. Chylomicron re...

BACKGROUND Homozygous or double heterozygous factor V Leiden and/or prothrombin G20210A is a rare inherited thrombophilic trait. Whether individuals with this genetic background have an increased risk of recurrent venous thrombosis is uncertain. METHODS AND RESULTS A case-control design within a large cohort of families with thrombophilia was chosen to calculate the risk of recurrent venous t...

In most flow systems, the rate of protein transfer from bulk solution to a macroscopic surface is site-dependent. In studies on surface-mediated protein conversion, this hampers the comparison of a proposed expression for the conversion process, such as the Michaelis-Menten equation, which actually measured overall conversion rates. However, the rotating disc is a classical example of a uniform...

Regulation of vascular homeostasis depends upon collaboration between cells of the vessel wall and blood coagulation system. A direct interaction between integrin a V b 3 on endothelial cells and smooth muscle cells and prothrombin, the pivotal proenzyme of the blood coagulation system, is demonstrated and activation of the integrin is required for receptor engagement. Evidence that prothrombin...

Background—Homozygous or double heterozygous factor V Leiden and/or prothrombin G20210A is a rare inherited thrombophilic trait. Whether individuals with this genetic background have an increased risk of recurrent venous thrombosis is uncertain. Methods and Results—A case-control design within a large cohort of families with thrombophilia was chosen to calculate the risk of recurrent venous thr...

on fibrinogen and oxalated plasma revealed that serum obtained from recently coagulated whole blood contains a factor which causes conversion of prothrombin to thrombin.” 2 Since fibrinogen could not be converted to fibrin in any significant degree by addition of fresh serum, and since the rapidity of coagulation of oxalated plasma by the serum factor was proportional to the prothrombin concent...

A new method for the specific assay of prothrombin is described which utilises Taipan snake venom and the chromogenic substrate Tos-Gly-Pro-Arg-pNA (Chromozym TH). The method is sensitive and reproducible and shows good correlation with a conventional specific assay for prothrombin. The reaction is dependent on calcium ions and phospholipid, and is therefore sensitive to the coumarin-induced pr...

CM. Comparison of plasma prothrombin and factor VII and urine prothrombin F1 concentrations in patients on long-term warfarin therapy and those in the initial phase. Am J Hematol 1998;57:193–9. 5. Johnston M, Harrison L, Moffat K, Willan A, Hirsh J. Reliability of the international normalized ratio for monitoring the induction phase of warfarin: comparison with the prothrombin time ratio. J Lab...

The physicochemical properties of bovine prothrombin and of bovine thrombin were previously described in this journal (1, 2). A derivative of bovine prothrombin, autoprothrombin II, has now been obtained in sufficient quality and quantity for study. In this report we describe some of the physicochemical properties of our product, including sedimentation coefficient, diffusion coefficient, intri...

BACKGROUND Prothrombin (FII) G20210A mutation and elevated plasma prothrombin activity are known risk factors for venous thrombosis. The risk of venous thrombosis among 19911G carriers of the prothrombin A19911G polymorphism has not been extensively investigated. OBJECTIVES AND METHODS We assessed prothrombin activity, FIIG20210A, and FIIA19911G polymorphisms in a large population-based case-...