Williamson, W. T. (North Carolina State of the University of North Carolina, Raleigh), S. B. Tove, and M. L. Speck. Extracellular proteinase of Streptococcus lactis. J. Bacteriol. 87:49-53. 1964.-Streptococcus lactis was shown to produce an extracellular proteolytic enzyme(s). A 120-fold purification of the proteinase was obtained from the cell-free culture medium by ammonium sulfate fractionat...

Plants respond to various stresses by expressing distinct sets of genes. The effects of multiple stresses on plants and their interactions are not well understood. We have discovered that salt stress causes the accumulation of proteinase inhibitors and the activation of other wound-related genes in tomato (Lycopersicon esculentum) plants. Salt stress was also found to enhance the plant's respon...

The production of extracellular proteinase by MY1049, a low-virulence mutant of Candida albicans, was compared with that of its wild-type parent, MY1044. Both strains were grown in a medium containing bovine serum albumin as a nitrogen source to induce proteinase production. Under these conditions, the proteinase activity per cell in the MY1049 cultures was the same as or higher than that of MY...

Degradation of the extracellular matrix leads to the release of fragments, which elicit biological responses distinct from intact molecules. We have reported that !1:Ser2091–Arg2108, a peptide derived from the !1-chain of laminin-1, triggers protein kinase C-dependent activation of MAPKerk1/2, leading to the up-regulation of macrophage urokinase type plasminogen activator and matrix metalloprot...

The nematodes like root-knot and cyst are plant-parasitic pest found in horticultural and agricultural crops. They do damages in the roots of plants as a result losses million tons of production. High cost of nematicides and environment safety concern has necessitated finding of some alternative methods. Under Integrated Pest Management (IPM) such problems are solving significantly by means of ...

Immunologic measurements of the serum concentration of prostate-specific antigen (PSA), an abundant prostatic-secreted serine proteinase, are frequently used to monitor patients with prostate cancer, though it has not been ascertained whether this immunoreactivity represents a PSA zymogen, the active proteinase, or PSA complexed to extracellular proteinase inhibitors. To characterize the PSA im...

A large, intracellular proteinase accumulated by inhibitors (PABI) was found in cultured mammalian cells as a large, multicatalytic proteinase with a greatly elevated concentration in the presence of small peptide proteinase inhibitors (Tsuji and Kurachi (1989) J. Biol. Chem. 264, 16093). Electron microscopic analysis showed that the tertiary structure of PABI highly resembled that of alpha 2-m...

BACKGROUND Sorghum types suitable for brewing and bioethanol production are required. The effect of sorghum type (white non-tannin versus white type II tannin) on free amino nitrogen (FAN) production from sorghum grain and malt using exogenous protease enzymes was investigated over extended incubation at moderate temperature (45 °C). RESULTS With grain in the absence of exogenous proteases, w...

Semi-alkaline proteinases having optimum pH between 8 and 9 of Streptomyces origin. have been reported as products of Streptomyces erythreus,3)Streptomyces fradiae4)and Streptomyces madurae.5)The proteolytic activity of all of the above semi-alkaline proteinases can be inactivated by addition of diisopropylfluorophosphate.We have recently isolated a new semi-alkaline proteinase from the culture...

fasciolosis, or liver fluke disease, caused by parasites of the genus fasciola is emerging as an important disease in man and animals, in the world and iran, particularly in nortern parts. the economical losses in domestic animals are considerable. in the recent decade there were two major outbreaks of human fasciolosis in the caspian region, northern part of iran with 7000-10000 infected cases...