The delicacy of food can be improved by employing flavor seasoning addition. purpose this study was to investigate the effect material combination oil palm mushrooms and snakehead fish drying temperature variation on seasonings' physical, chemical, microbiological, organoleptic properties. experiment employed a Completely Randomized Design (CRD) consisting two factors. first factor ratio 25%: 7...

Effective isolation and purification of protein is a great challenge nowadays. The key aspect stability solubility, which primarily depend on structure its amino acid sequence. Manipulations with pH ionic strength are the first at tempts to increase solubility. Different additives that allowed or prohibited in food industry applied for overcoming aggregation. Sugars, polyhydric alcohols acids m...

Silkworm (Bombyx mori) has been reported to exhibit diverse health benefits. A comparative study was conducted evaluate the physicochemical (colour, water activity, pH, solubility, proximate, mineral, amino acid, and fatty acid composition) microbiological qualities of Bombyx mori silkworm powder produced from two developmental stages (larvae pupae). pupae (SPP) had significantly higher (p<0...

Recent studies suggest that reducing the induction temperature would improve the quality of some recombinant inclusion bodies (IB) by providing a native-like secondary structure and leading to an improvement in protein recovery. This study focused on optimizing the solubilization condition of Reteplase, a recombinant protein with 9 disulfide bonds. The influence of lowering induction temperatur...

Recent studies suggest that reducing the induction temperature would improve the quality of some recombinant inclusion bodies (IB) by providing a native-like secondary structure and leading to an improvement in protein recovery. This study focused on optimizing the solubilization condition of Reteplase, a recombinant protein with 9 disulfide bonds. The influence of lowering induction temperatur...

Low solubility of proteins overexpressed in E. coli is a frequent problem in high-throughput structural genomics. To improve solubility of proteins from mesophilic Shewanella oneidensis MR-1 and thermophilic Clostridium thermocellum JW20, an approach was attempted that included a fusion of the target protein to a maltose-binding protein (MBP) and a decrease of induction temperature. The MBP was...

This paper deals with the investigation of the impact of the heat treatment of reconstituted skim milk conducted at different temperatures, and the adding of demineralized whey on the protein solubility, soluble protein composition and interactions involved between proteins in a chemical complex. Commercial skim milk has been reconstituted and heat treated at 75°C, 85°C and 90°C for 20 minutes....

BACKGROUND The growing field of proteomics and systems biology is resulting in an ever increasing demand for purified recombinant proteins for structural and functional studies. Here, we show a systematic approach to successfully express a full-length protein of interest by using cell-free and cell-based expression systems. RESULTS In a pre-screen, we evaluated the expression of 960 human ful...

Low molecular size additives such as L-arginine and the redox compounds have been used both in the culturemedium and in vitro refolding to increase recombinant proteins production. Additives increase proteinrefolding and yield of active proteins by suppressing aggregate formation or enhancing refolding process.In this work, a comparative study was performed on refolding of rec...

Membrane and nuclear proteins of poor solubility have been separated by high resolution two-dimensional (2-D) gel electrophoresis. Isoelectric focusing with immobilized pH gradients leads to severe quantitative losses of proteins in the resulting 2-D map, although the resolution is usually high. Protein solubility could be improved by using denaturing solutions containing various detergents and...