background the 30 kda major secretory protein of mycobacterium tuberculosis (antigen 85b) is a primary vaccine candidate. this secreted antigen induces a protective immune response and stimulates the production of ifn-γ in animal models. objectives the aim of this study was cloning and expression of ag 85b of m. tuberculosis in escherichia coli. materials and methods to produce recombinant ag85...

1 Institute of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China 2 Clinical Research, Diabetes and Endocrinology, Merck Research Laboratories, 126 East Lincoln Avenue, RY34B-148, Rahway, NJ 07065-0900, USA 3Department of Nutritional Science, Rutgers University, New Brunswick, NJ 08901, USA 4Division of Endocrinology, Metabolism an...

Aeromonas hydrophila is a gram-negative bacterium which associated with gastrointestinal diseases and septicaemia. This pathogenic bacterium has several virulence factors ranging from pili to the excreted protein which called (Aerolysin) with minor and major effects, respectively. Additionally, Aeromonas hydrophila is a widely distributed bacterium that commonly causes ulcers in cyprinid fish s...

bovine ephemeral fever (bef) is an acute epidemic disease in cattle and water buffalo, spanning tropical and subtropical zones of asia, australia, and africa. in recent years, bef has been distributed in many provinces of iran and caused economic losses. in this study the sequence encoding the g1 epitope of bovine ephemeral fever virus (befv) g glycoprotein was amplified by pcr, ligated into th...

objective(s):multi-epitopic protein vaccines and direction of vaccine delivery to dendritic cells (dcs) are promising approaches for enhancing immune responses against mutable pathogens. escherichia coli is current host for expression of recombinant proteins, and it is important to optimize expression condition. the aim of this study was the optimization of multi-epitopic hiv-1 tat/pol/gag/env ...

Effects of protein supplementation, with and without starch on nutritional performance metabolic characteristics cattle fed low- medium-quality tropical forages were evaluated using 4 cannulated steers distributed according to a × Latin square. Experimental periods divided into two subperiods. In the first subperiod, animals received low-quality hay medium-quality. Supplementation schemes in se...