background and objectives: the linear epitopes of gp46-i, gp46-ii, gp21 and p19 are used in diagnosis of htlv-i/-ii infections. the aims of this study was to obtain high-level expression and purification of recombinant antigen (ra) containing these epitopes. large-scale preparation of such antigen probably worths for diagnostic purpose. materials and methods: the synthetic dna encoding ra was s...

Background: The immunogenic Brucella abortus ribosomal protein L7/L12 is a promising candidate antigen for the development of subunit vaccines against brucellosis. Objective: This study was aimed to evaluate the protection of recombinant Human Serum Albumin (HAS)-L7/L12 fusion protein in Balb/c mice. Methods: The amplified L7/L12 gene was cloned in pYHSA5 vector, pYHSA5-L7/L12 construct was tra...

Introduction: The Nef accessory protein is an attractive antigenic candidate in the development of HIV-1 DNA- or protein-based vaccines. The most crucial disadvantage of DNA and protein-based vaccines is their low immunogenicity, which can be improved by cell-penetrating peptides (CPPs) as effective carrier molecules. Methods: In this study, the HIV-1 Nef protein was generated in the Escherichi...

the tlr4 gene structure as the main receptor for lipopolysaccharide recognition of gram-negative bacteria was investigated in two strains of iranian commercial arian line and west azerbaijan native chicks and its expression was studied in some major organs. blood samples of the 120 birds were taken and total dnas extracted. then, the target gene was sequenced using four pairs of primers on four...

the pituitary-specific transcription factor (pit-1) gene is a candidate gene for growth, carcass and also for milk yield traits. in dairy farm animals, the main goal of the selection is the improvement of milk yield and composition. the genes of milk proteins and hormones are excellent candidate genes for linkage analysis with quantitative trait loci (qtl) because of their biological significan...

Malaria vaccination is an essential approach to combat malaria. One major protein studied for vaccine development Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP1). It contains several important domains malaria pathogenesis. The binding of Cysteine-rich interdomain region ?1 (CIDR?1) PfEMP1 endothelial C receptor (EPCR) associated with cerebral malaria, while CIDR?1 CD36 has been co...

Objective(s): Acinetobacter baumannii is one the most dangerous opportunistic pathogens in hospitalized infections. This bacterium is resistant to 90% of commercial antibiotics. Therefore, developing new strategies to cure A. baumannii-infections is urgent. The DNA vaccines new approach in which the immunogen can be directly expressed inside the target cells through cl...

Introduction: The development of an effective vaccine against Hepatitis C virus (HCV) is still a target of intensive vaccine research. The HCV envelope proteins E1 and E2 which can induce broadly neutralizing antibodies are the major candidate for this purpose. Different types of expression systems have been used to express these glycoproteins. In this study, an expression system using Pichia p...