the aim of the study was to describe the urinary electrophoretic pattern of dogs with heartworm disease.urine samples from 15 heartworm-infected and 15 healthy dogs were taken. urinary specific gravity, urinaryprotein concentration and the urine protein/creatinine (u p/c) ratio were determined. urine proteins werefractionated using sds-page. results showed statistically significant differences ...

seed protein usually is not sensitive to environmental fluctuations; its banding pattern is very stable which advocated for cultivars identification purpose in crop. this study was planned to study effect of water deficit on protein content and protein banding pattern of wheat genotypes. in this study five wheat genotypes were planted in early november, growing season of 2008-2009. the experime...

At various postnatal stages, intestinal epithelial cells were isolated sequentially from villus tip to crypt base by successive EDTA treatments. According to the localization of marker enzymic activities, isolated cells were pooled into three cell compartments: villus (V), lower villus and upper crypt (VC) and crypt (C). Purified brush-border-membrane proteins were separated by 7.5%-polyacrylam...

Anaplasma marginale is the etiological agent of a hemolytic disease of cattle, known as anaplasmosis. The organism appears as a marginal inclusion in parasitized erythrocytes, but certain isolates also have bands associated with the inclusion. Inclusions and associated bands in parasitized erythrocytes in the liver and peripheral circulation were studied by light microscope cytochemistry and el...

The biosynthesis of 14 protein bands from 50S ribosomes was studied during the outgrowth of Bacillus subtilis W23 spores. The time at which the synthesis of each protein band begins and the patterns of synthesis that each displays were determined by comparing the incorporation of (3)H-phenylalanine during five intervals throughout the first hour of outgrowth with the incorporation of (14)C-phen...

Polyacrylamide·.g~1 electrophoresis with glycine buffer (pH 9.2) containing 8 M urca separated soybean whey proteins into at least 24 bands. In contrast, ultracentrifugation indicated only 2 fractions; moving-boundary electrophoresis, 8-9 components; and column chromatography, 13 or more proteins. A prominent, fast-moving band in the gel pattern appeared to be identical to crystalline soybean t...

Obscurin is a multidomain protein composed of adhesion and signaling domains that plays key roles in the organization of contractile and membrane structures in striated muscles. Overexpression of the second immunoglobulin domain of obscurin (Ig2) in developing myotubes inhibits the assembly of A- and M-bands, but not Z-disks or I-bands. This effect is mediated by the direct interaction of the I...

One of the most commonly observed surface structures on the prokaryotic cell envelopes is monomolecular crystalline array of proteinaceous subunits termed Surface Layers or S-layers. Due their self assembly ability and the highly ordered, regular structure down to the nanometer scale, S-layers were demonstrated to possess a great potential for nanobiotechnological applications. Some probiotic b...