introduction: in recent years, influenza viruses have caused moderate to severe infections all around the world while so far there is no influenza vaccine that can protect people with only one dose of injection. in this regard, producing a universal vaccine based on virus-like-particles (vlp) could be an ideal approach.  methods: in this study, the full-length orf of influenza hemagglutinin (ha...

results cloning was confirmed by pcr and restriction digestion. rt-pcr and sds-page represented expression of kgf in e. coli. the optimized expression was achieved 16 hours after induction with 0.3 mm iptg at 37°c in luria broth (lb) containing kanamycin. the 18 kda protein was confirmed by western blotting, using anti-his antibodies. conclusions the result of the present study indicated that e...

interference with microtubule polymerization results in cell cycle arrest leading to cell death. colchicine is a well-known microtubule polymerization inhibitor which does so by binding to a specific site on tubulin. a set of 3',4'-bis (substituted phenyl)-4'h-spiro[indene-2,5'-isoxazol]-1(3h)-one derivatives with known antiproliferative activities were evaluated for their t...

background: recently, we have shown that peroxisomal protein expression was induced upon retinoic acid treatment in mouse embryonic stem cells during the process of neurogenesis. thus, characterization of the respective promoter could elucidate the molecular aspects of transcriptional regulation of this gene. methods: using the conventional software programs for promoter prediction, a putative ...

background in order to detect nephropathy, measurement of total (24 hrs) urinary albumin or albumin/creatinin ratio in random urine samples is being recommended. but methods of albumin measurement are not available in all laboratories and also cost about 6 times more than that of urinary total protein measurement. objectives this study was performed to determine appropriate cut off point in 24 ...

conclusion our findings confirmed that a prokaryotic expression system of rureb229-561 was successfully constructed.the results of sds-page showed that our constructed prokaryotic expression system pet28a- ureb229-561 -bl21de3 efficiently produces target recombinant protein in the form of dissoluble inclusion body. therefore we can suggest that these epitopes can effectively be a vaccine candid...

protein memories are novel technologies which use the hidden abilities of bacteria proteins and biological molecules in order to the electronic processes. four decades before baceriorhdopsin ( br) protein was extracted from the membrane of halobacerinum salina rum found in high salt water depositions after its original discovery in 1967. in this study, for immobilizing the protein a film has be...

background hepatitis c virus (hcv) is the major cause of chronic liver disease. hcv is a single stranded positive sense rna of approximately 9.6 kb. because of high conservativeness of 5΄untranslated region of hcv genome, it is widely used for virus genotyping. different methods are used for the virus genotyping, but all involve some difficulties. objectives the aim of the present study was to ...

in present study different protein measurement methods are evaluated on the allergenic extract of chenopodium album pollen, which was previously prepared in our laboratory and applied in skin prick testing in comparison with a commer¬cial extract. the protein content of similar amounts of these two extracts which had caused similar skin reactions were measured with different protein assays such...

objective(s):this study aims at exploring cytotoxic activity of different peptides derived from vsvg protein against mcf-7 and mda-mb-231 breast cancer cell lines and human embryonic kidney normal cell (hek 293).    materials and methods: the anticp web server was used to predict anticancer peptides. the cytotoxic activity of peptides with high score (p26, p7) and low score (p19) was examined b...