Penelitian ini bertujuan mengeksplorasi senyawa kimia kulit kopi sebagai anti Covid-19 (anti-3C-like protease). Analisis in silico dilakukan dengan tahapan pengambilan struktur dan protein, preparasi penginteraksian protein 3CL-Pro. Docking program Molegro virtual Docker 5 divisualisasi Discovery Studio versi 21.1.1. Senyawa yang terkandung dalam kopi, yaitu quinic acid, gluconic acid maupun fe...

We quantitatively determined whether the selective phosphodiesterase (PDE) inhibitor, rolipram, inhibits changes in the adhesion molecules CD11b and L-selectin on platelet-activating factor (PAF)-stimulated human neutrophils and eosinophils in vitro. Incubations were performed in human whole blood obtained from healthy volunteers, to restrict activation by purification procedures and to simulat...

Objective—This study was conducted to examination whether Ginkgo biloba extract (GBE), a Chinese herb with antioxidant activity, could reduce cytokine-induced monocyte/human aortic endothelial cell (HAEC) interaction, a pivotal early event in atherogenesis. Methods and Results—Pretreatment of HAECs with GBE (50 and 100 g/mL for 18 hours) significantly suppressed cellular binding between the hum...

Alcohol dehydrogenase (ADH), which oxidizes ethanol into acetaldehyde, exacerbates ethanol-induced cardiac depression, although the mechanism of action remains unclear. This study was designed to examine the impact of antioxidant catalase (CAT) on cardiac contractile response to ethanol and activation of stress signaling. ADH-CAT double transgenic mice were generated by crossing CAT and ADH lin...

in this study, the effect of accelerated ripening with encapsulated protease and lipase on physiochemical properties such as fat and moisture, texture and microstructure of brined cheese was investigated. addition of lipase increases hardness by reducing fat globules diameter and increasing casein matrix junction. it also decreases brittleness by fat degradation which is responsible for plastic...

Cell growth inhibitions on human cancer cell cultures were determined for the indole sulphur-containing phytoalexins cyclobrassinin, brassilexin (previously isolated from vegetables of the Cruciferae family) and their synthetic analogues 5-methoxybrassilexin and homocyclobrassinin. The most biologically active of these products is brassilexin (LD50 = 8 micrograms/ml).

Using as a template the crystal structure of SARS-CoV-2 main protease, we developed pharmacophore model functional centers protease inhibitor-binding pocket. With this model, conducted data mining conformational database FDA-approved drugs. This search brought 64 compounds that can be potential inhibitors protease. The conformations these undergone 3D fingerprint similarity clusterization. Then...

the aim of this study was to clone the serine alkaline protease-encoding gene from bacillus subtilis 168. this protease, which can have many applications especially in detergent, may be industrially an important enzyme. for the amplification of the gene, pcr was performed with a pair of primers specifically designed for this purpose. electrophoresis of the pcr product showed the expected band o...

This study investigates the cumulative effects of lifetime traumatization on dream activities and the extent to which potentially traumatic events, dream variables, and inhibitory functions can predict the severities of psychopathological symptoms. The sample consisted of 564 young adults, whose dream experiences, trauma history, ego functions, and symptom manifestations were assessed using the...

Background: The novel coronavirus SARS-CoV-2 has severely affected the health and economy of several countries. Multiple studies are in progress to design therapeutics against potential target proteins SARS-CoV-2, including 3CL protease, an essential protein for virus replication. Materials & methods: In this study we employed deep neural network-based generative predictive models de novo s...