background: sperm preparation is a fundamental step in art and attempt for developing new methods to be continued. objective: to evaluate the efficacy of upstream method for separation of human spermatozoa and its effects on sperm parameters including: motility morphology and concentration. materials and methods: semen samples from 17 men who referred to infertility clinic for evaluating their ...

Abstract Recent reports suggest that mobile phone radiation may diminish male fertility. However, the effects of this radiation on human spermatozoa are largely unknown. The present study examined effects of the radiation on induction of apoptosis-related properties in human spermatozoa. Ejaculated, density-purified, highly motile human spermatozoa were exposed to mobile phone radiation at spec...

The acrosome reaction by spermatozoa is an indispensable prerequisite for fertilization, and zonae pellucidae of human oocytes induce this reaction in the human spermatozoa attached to them. Human spermatozoa can attach to the zona pellucida of hamster oocytes when the oocytes have been treated with trypsin. We examined whether or not trypsin-treated hamster zona pellucida induces the acrosome ...

It is possible and clinically relevant to distinguish between slow and rapid progressive spermatozoa in basic semen analysis. This is discussed in light of the different purposes of semen analysis for the subfertile couple and the male patient. The two groups of progressive spermatozoa should be distinguished to help ensure that pertinent information available in the semen sample is not neglected.

OBJECTIVE To evaluate the role of hyaluronic acid (HA) for sperm selection before intracytoplasmic sperm injection (ICSI). DESIGN Three prospective studies. SETTING Private assisted reproduction center in Italy. PATIENT(S) Study 1: 20 men. Study 2: 15 men. Study 3: 206 couples treated with ICSI on a limited number of oocytes per patient (1-3) in accordance with Italian IVF law. INTERVEN...

ObjectiveMetabolomic profiling of seminal plasma has been suggested as a possible approach for fast and non-invasive male infertility evaluation diagnosis. However, metabolomics profiles in normozoospermic men have not thoroughly investigated, the influence ejaculation-abstinence described.To provide interim reference values find associations between human length period men.Study designSemen sa...

The aim of this study was to compare computer assisted sperm analysis (CASA) results frozen thawed bull semen using three different chambers. Sixty samples were (37°C; 30 sec), extended in PBS (30×106 spermatozoa/mL; 37°C) and incubated 2 min). Each sample analyzed by CASA [total motility, progressive (pro)/ non-progressive/rapid/medium/slow movement spermatozoa, VCL, VSL, VAP, ALH, BCF, LIN, S...

Background: The lipid peroxides and their degradation products are highly toxic to spermatozoa and may play a major role in sperm dysfunction. The purpose of this study was to determine the level of lipid peroxidation as indicated by malondialdehyde (MDA) in the spermatozoa and seminal plasma of asthenozoospermic and normozoospermic males. In addition, correlation of lipid peroxidation with mot...