X-ray crystallography provides important insights into structure-function relationship in biomolecules. However, protein crystals are usually hard to obtain which hinders our understanding of multiple important processes. Crystallization requires large amount of protein sample, whereas recombinant proteins are often unstable or insoluble. Green fluorescent protein (GFP) fusion is one of the app...

Thermal stability of the thermolysin-like protease produced by Bacillus stearothermophilus (TLP-ste) is highly dependent on calcium at concentrations in the millimolar range. We describe the rational design and production of a fully active TLP-ste variant whose stability is only slightly dependent on calcium concentration.

Nanofluids, colloidal suspensions consisting of base fluids and nanoparticles, are a new generation of engineering working fluids. Nanofluids have shown great potential in heat/mass transfer applications. However, their practical applications are limited by the high production cost and low stability. In this study, a low-cost agricultural waste, rice husk ash (RHA), was used as a silicon source...

using enrichment procedures, 45 organic solvent-tolerant lipase producer bacterial strains were screened from areas contaminated by oil and organic solvents. among the strains, 15 isolates exhibited extreme stability toward organic solvents and high lipolytic activity. the neb-1 isolate which was later identified as   pseudomonas sp. strain neb-1 by biochemical tests and 16s rdna gene sequ...

Composting is one of the best ways to properly manage the high production of sewage sludge with the aim of obtaining sanitized products with a stable organic matter. The evolution of the stability of a compost consisting of sewage sludge, straw and sawdust, developed under full-scale conditions, was analyzed. The stability was assessed by a respirometric assay performed for 30 days, where the O...

The dynamics of systems with stochastically varying time delays are investigated in this paper. It is shown that the mean dynamics can be used to derive necessary conditions for the stability of equilibria of the stochastic system. Moreover, the second moment dynamics can be used to derive sufficient conditions for almost sure stability of equilibria. The results are summarized using stability ...

Rapid progress in disease biomarker discovery has increased the need for robust detection technologies. In the past several years, the designs of many immunoaffinity reagents have focused on lowering costs and improving specificity while also promoting stability. Antibody fragments (scFvs) have long been displayed on the surface of yeast and phage libraries for selection; however, the stable pr...

The dynamics of systems with stochastically varying time delays are investigated in this paper. It is shown that the mean dynamics can be used to derive necessary conditions for the stability of equilibria of the stochastic system. Moreover, the second moment dynamics can be used to derive sufficient conditions for almost sure stability of equilibria. The results are summarized using stability ...

The establishment of erythromycin production within the heterologous host E. coli marked an accomplishment in genetic transfer capacity. Namely, over 20 genes and 50 kb of DNA was introduced to E. coli for successful heterologous biosynthetic reconstitution. However, the prospect for production levels that approach those of the native host requires the application of engineering tools associate...

Stability studies play a critical role in assuring product quality at all points in the vaccine life cycle. These studies used to determine vaccine expiry date and vaccine efficacy. Accelerated stability and long term stability study performed for three batches of trivalent oral poliomyelitis vaccine (OPV) and three batches of monovalent OPV (type1) manufactured by Razi institute. After samplin...