Chromosomes of the cellular slime mold Dictyostelium discoideum were fractionated on three pulse field gel electrophoresis systems (pulse field, orthogonal field and C.H.E.F. (Contour-clamped Homogeneous Electric Fields] into a series of 13 bands ranging from 0.1 Mb to over 2 Mb in size. Since this organism has only seven chromosomes (estimated to be 1-10 Mb), and -90 copies of an 88-kilobase l...

During late M and early G(1), MCM2-7 assembles and is loaded onto chromatin in the final step of prereplicative complex (pre-RC) formation. However, the regulation of MCM assembly remains poorly understood. Cyclin-dependent kinase (CDK)-dependent phosphorylation contributes to DNA replication by initially activating pre-RCs and subsequently inhibiting refiring of origins during S and M phases, ...

west part of iran is considered as one of the center of diversity for aegilops triunsialis, therefore, study of its genetic diversity in this area is extremely important regarding evolution of this plant. the genetic variation among 40 accessions of ae.triunsialis collected from west of iran were studied using 12 issr markers. dna was extracted, concentrations adjusted and five samples from eac...

Covalently closed intracellular and viral simian virus 40 (SV40) deoxyribonucleic acid (DNA) were separately isolated from infected African green monkey cells (BSC-1) grown in culture. The two DNA species form overlapping bands centered at different positions in a propidium di-iodide-cesium chloride (PDI-CsCl) buoyant density gradient capable of separating closed DNA species with different supe...

A metastable β Ti alloy was additively manufactured by laser powder bed fusion (LPBF). Tensile testing along the build direction of as-LPBF material (LPBF-0°) revealed significant work softening immediately following yielding with no uniform deformation. By contrast, substantial hardening and elongation well over 10% were achieved perpendicular to (LPBF-90°). Similar effects obtained in after s...

PURPOSE Iontophoresis has been used for drug delivery across the cornea for many years. We sought to test whether small charged dyes and DNA can be transferred across human sclera by an electric field. METHODS Full-thickness human scleral fragments were embedded vertically in an agarose gel and positioned to completely span individual gel lanes. The scleral fragments were located approximatel...

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to use remote sensing data effectively, one must understand the spectral characteristics of the particular features under investigations. to study the relationship between soil salinity and soil reflectance, four multispectral scanners (mss) and seven thematic mappers (tm bands) over the ardakan playa located on the central iranian desert margins were selected. in this study soil map, soil ...