Previous studies have shown that Gardnerella vaginalis can utilize iron-loaded human lactoferrin as a sole source of iron. In this study, G. vaginalis cells were shown to bind digoxigenin (DIG)-labeled human lactoferrin in a dot blot assay. Using the DIG-labeled human lactoferrin, a 120-kDa human lactoferrin-binding protein was detected by Western blot analysis of G. vaginalis proteins. The lac...

Trichomonas vaginalis is a parasitic protozoan causing the most common human sexually transmitted disease (STD), trichomoniasis. The exact mechanism of its pathogenesis is still obscure. Alpha-enolase plays a pivotal role in the host-pathogen interaction, and as a surface receptor of several protists mediating plasminogen binding. In view of identifying plasminogen binding sites of T. vaginalis...

Trichomonas vaginalis and Tritrichomonas foetus are pathogens that parasitise, respectively, human and bovine urogenital tracts causing disease. Using LC-MS, reference metabolomic profiles were obtained for both species and stable isotope labelling with D-[U-13C6] glucose was used to analyse central carbon metabolism. This facilitated a comparison of the metabolic pathways of T. vaginalis and T...

Meconium periorchitis is a rare disorder caused by fetal meconium peritonitis, with subsequent passage of meconium into the scrotum via a patent processus vaginalis. To date, clinical significance of meconium periorchitis for the prenatal diagnosis of meconium peritonitis and prediction for postnatal surgery remains to be determined. We present a clinical course of a fetus presenting with mecon...

Neutrophils play an important role in the human immune system for protection against such microorganisms as a protozoan parasite, Trichomonas vaginalis; however, the precise role of neutrophils in the pathogenesis of trichomoniasis is still unknown. Moreover, it is thought that trichomonal lysates and excretory-secretory products (ESP), as well as live T. vaginalis, could possibly interact with...

Trichomonas vaginalis induces apoptosis in host cells through various mechanisms; however, little is known about the relationship between apoptosis, reactive oxygen species (ROS), and NF-κB signaling pathways in the cervical mucosal epithelium. Here, we evaluated apoptotic events, ROS production, and NF-κB activity in T. vaginalis-treated cervical mucosal epithelial SiHa cells, with or without ...

OBJECTIVE In this study, the cytopathic effects of Trichomonas vaginalis were investigated in L929 mouse fibroblast cell cultures (FCC) under different conditions: only parasite, or coexistence with Candida albicans and Escherichia coli. METHODS The parasite was isolated from a symptomatic patient and cultured in Cysteine-Peptone-Liver infusion Maltose medium (CPLM). C. albicans strain 10235 ...

Trichomoniasis, caused by the parasitic protozoan Trichomonas vaginalis, is the most common non-viral sexually-transmitted disease, and there can be severe complications from trichomoniasis. Antibiotic resistance in T. vaginalis is increasing, but there are currently no alternatives treatment options. There is a need to discover and develop new chemotherapeutic alternatives. Plant-derived natur...

Variables affecting the adherence of Gardnerella vaginalis to human vaginal epithelial cells were examined in vitro. Adherence depended on pH, with maximum attachment occurring between pH 5 and pH 6. Preincubation of the bacteria at 56 degrees C for 30 minutes and ultraviolet irradiation resulted in a noticeable decrease in adherence. In contrast, adherence was not altered by preincubating the ...

Gardnerella vaginalis was isolated from 22 (38%) of 58 semen samples obtained from men attending an infertility clinic. Counts ranged from 1.2 X 10(3) to greater than 10(7) colony forming units (cfu)/ml. There was no association between the isolation of G vaginalis and the sperm count. Twenty (34.4%) samples contained non-sporing anaerobes and nine (15.5%) both anaerobes and G vaginalis. The in...